Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3519748..3520331 Replicon chromosome
Accession NZ_CP026831
Organism Shigella dysenteriae strain ATCC 12039

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag C1P71_RS19330 Protein ID WP_096305941.1
Coordinates 3519996..3520331 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag C1P71_RS19325 Protein ID WP_000581937.1
Coordinates 3519748..3519996 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P71_RS19315 (3516087) 3516087..3517388 + 1302 WP_128881085.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
C1P71_RS19320 (3517436) 3517436..3519670 + 2235 WP_128881086.1 GTP diphosphokinase -
C1P71_RS19325 (3519748) 3519748..3519996 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
C1P71_RS19330 (3519996) 3519996..3520331 + 336 WP_096305941.1 endoribonuclease MazF Toxin
C1P71_RS19335 (3520403) 3520403..3521194 + 792 WP_128881087.1 nucleoside triphosphate pyrophosphohydrolase -
C1P71_RS19340 (3521422) 3521422..3523059 + 1638 WP_128881088.1 CTP synthase (glutamine hydrolyzing) -
C1P71_RS19345 (3523146) 3523146..3524444 + 1299 WP_000036723.1 phosphopyruvate hydratase -
C1P71_RS19350 (3524504) 3524504..3524923 - 420 Protein_3508 hypothetical protein -
C1P71_RS19355 (3524986) 3524986..3525246 + 261 Protein_3509 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12112.07 Da        Isoelectric Point: 8.2618

>T96302 WP_096305941.1 NZ_CP026831:3519996-3520331 [Shigella dysenteriae]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCIPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T96302 NZ_CP026831:3519996-3520331 [Shigella dysenteriae]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTATTCCTTGTACAACAC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAGCTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT96302 WP_000581937.1 NZ_CP026831:3519748-3519996 [Shigella dysenteriae]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT96302 NZ_CP026831:3519748-3519996 [Shigella dysenteriae]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTACGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References