Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3341607..3342225 Replicon chromosome
Accession NZ_CP026831
Organism Shigella dysenteriae strain ATCC 12039

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C1P71_RS18485 Protein ID WP_001291435.1
Coordinates 3342007..3342225 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C1P71_RS18480 Protein ID WP_000344800.1
Coordinates 3341607..3341981 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P71_RS18470 (3336994) 3336994..3340143 + 3150 WP_128881017.1 efflux RND transporter permease AcrB -
C1P71_RS18475 (3340722) 3340722..3341566 + 845 WP_128879749.1 IS5-like element ISSfl7 family transposase -
C1P71_RS18480 (3341607) 3341607..3341981 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C1P71_RS18485 (3342007) 3342007..3342225 + 219 WP_001291435.1 HHA domain-containing protein Toxin
C1P71_RS18490 (3342397) 3342397..3342948 + 552 WP_000102564.1 maltose O-acetyltransferase -
C1P71_RS18495 (3343064) 3343064..3343534 + 471 WP_000136192.1 YlaC family protein -
C1P71_RS18500 (3343698) 3343698..3345248 + 1551 Protein_3343 cyclic-guanylate-specific phosphodiesterase PdeB -
C1P71_RS18505 (3345290) 3345290..3345643 - 354 WP_000878140.1 DUF1428 domain-containing protein -
C1P71_RS18515 (3346022) 3346022..3346333 + 312 WP_000409911.1 MGMT family protein -
C1P71_RS18520 (3346364) 3346364..3346936 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3341051..3341566 515


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T96301 WP_001291435.1 NZ_CP026831:3342007-3342225 [Shigella dysenteriae]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T96301 NZ_CP026831:3342007-3342225 [Shigella dysenteriae]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT96301 WP_000344800.1 NZ_CP026831:3341607-3341981 [Shigella dysenteriae]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT96301 NZ_CP026831:3341607-3341981 [Shigella dysenteriae]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References