Detailed information of TA system TA096291

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 351695..352271
• Replicon: chromosome
• Accession: NZ_CP026831
• Organism: Shigella dysenteriae strain ATCC 12039

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: C1P71_RS02990
• Protein ID: WP_128879866.1
• Coordinates: 351695..351982 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A2U2VE39
• Locus tag: C1P71_RS02995
• Protein ID: WP_000063158.1
• Coordinates: 351969..352271 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P71_RS02975 (347238)	347238..348659	-	1422	WP_128879863.1	restriction endonuclease subunit S	-
C1P71_RS02980 (348649)	348649..350268	-	1620	WP_128879864.1	class I SAM-dependent DNA methyltransferase	-
C1P71_RS02985 (350332)	350332..351498	-	1167	WP_128879865.1	restriction endonuclease	-
C1P71_RS02990 (351695)	351695..351982	+	288	WP_128879866.1	BrnT family toxin	Toxin
C1P71_RS02995 (351969)	351969..352271	+	303	WP_000063158.1	BrnA antitoxin family protein	Antitoxin
C1P71_RS03000 (352305)	352305..353261	-	957	WP_128879867.1	GTPase	-
C1P71_RS03005 (353272)	353272..353475	-	204	WP_128879868.1	YbdD/YjiX family protein	-
C1P71_RS03010 (353525)	353525..355675	-	2151	WP_001298933.1	pyruvate/proton symporter BtsT	-
C1P71_RS03015 (356065)	356065..356577	-	513	WP_128879869.1	4-hydroxyphenylacetate 3-monooxygenase reductase subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11237.74 Da
• Isoelectric Point: 7.4022

>T96291 WP_128879866.1 NZ_CP026831:351695-351982 [Shigella dysenteriae]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDEPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T96291 NZ_CP026831:351695-351982 [Shigella dysenteriae]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGAACCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCT
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11537.99 Da
• Isoelectric Point: 9.4646

>AT96291 WP_000063158.1 NZ_CP026831:351969-352271 [Shigella dysenteriae]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDSDVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT96291 NZ_CP026831:351969-352271 [Shigella dysenteriae]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCCTCGGTGCGTATTGATTCTGATGTGATGGAATGGTTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A2U2VE39