Detailed information of TA system TA096288

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 111330..111955
• Replicon: plasmid unnamed
• Accession: NZ_CP026830
• Organism: Shigella dysenteriae strain ATCC 12039

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C1P71_RS00740
• Protein ID: WP_128879691.1
• Coordinates: 111330..111728 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q31SG2
• Locus tag: C1P71_RS00745
• Protein ID: WP_000450530.1
• Coordinates: 111728..111955 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P71_RS00705 (106970)	106970..107510	-	541	Protein_134	transposase	-
C1P71_RS00710 (107625)	107625..108263	-	639	WP_000607009.1	type III secretion system effector OspI	-
C1P71_RS00715 (108422)	108422..108892	-	471	Protein_136	IS1 family transposase	-
C1P71_RS00725 (109264)	109264..109443	+	180	Protein_137	helix-turn-helix domain-containing protein	-
C1P71_RS00730 (109532)	109532..110760	+	1229	WP_088895425.1	IS3-like element IS2 family transposase	-
C1P71_RS00735 (110869)	110869..111321	+	453	Protein_139	conjugal transfer protein TraD	-
C1P71_RS00740 (111330)	111330..111728	-	399	WP_128879691.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C1P71_RS00745 (111728)	111728..111955	-	228	WP_000450530.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ospC2 / ospE2 / ospC1 / ospD3/senA / ospG / ipaH9.8 / virA / icsA/virG / ospI / nleE / icsP/sopA / ospB / ospD2 / ospF / ospD1 / ipgB2 / ipaH4.5 / ipaH9.8	1..181100	181100

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14788.03 Da
• Isoelectric Point: 7.8604

>T96288 WP_128879691.1 NZ_CP026830:c111728-111330 [Shigella dysenteriae]
MLKFMLDTNICIFTIKNKPASVRESFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAAIHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRTEDWS

Nucleotide

• Length: 399 bp

>T96288 NZ_CP026830:c111728-111330 [Shigella dysenteriae]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGCGTCAGGGAGAGTTTTAA
CCTTAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCCGCTGCAGCCATACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAACTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8535.59 Da
• Isoelectric Point: 4.7513

>AT96288 WP_000450530.1 NZ_CP026830:c111955-111728 [Shigella dysenteriae]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT96288 NZ_CP026830:c111955-111728 [Shigella dysenteriae]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: Q31SG2