Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 137883..138442 Replicon plasmid unnamed
Accession NZ_CP026829
Organism Shigella dysenteriae strain ATCC 12037

Toxin (Protein)


Gene name relE Uniprot ID D2AJT2
Locus tag C1P70_RS25455 Protein ID WP_000421262.1
Coordinates 137883..138158 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q327A8
Locus tag C1P70_RS25460 Protein ID WP_001178088.1
Coordinates 138158..138442 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P70_RS25425 132884..134097 + 1214 Protein_167 IS3 family transposase -
C1P70_RS25435 134161..135291 + 1131 WP_050546159.1 IS91 family transposase -
C1P70_RS25440 135486..136577 - 1092 Protein_169 IS66 family transposase -
C1P70_RS25445 136585..137152 + 568 Protein_170 plasmid segregation protein ParM -
C1P70_RS26995 137155..137346 + 192 WP_252988941.1 plasmid partitioning/stability family protein -
C1P70_RS26410 137614..137751 + 138 WP_000766699.1 hypothetical protein -
C1P70_RS25455 137883..138158 - 276 WP_000421262.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C1P70_RS25460 138158..138442 - 285 WP_001178088.1 ribbon-helix-helix domain-containing protein Antitoxin
C1P70_RS25470 138894..139145 + 252 WP_000433928.1 transporter -
C1P70_RS25475 139195..139404 + 210 Protein_176 peptidoglycan-binding protein -
C1P70_RS27000 139486..139587 - 102 Protein_177 ATP-binding protein -
C1P70_RS25480 139739..140095 + 357 WP_000239757.1 transposase -
C1P70_RS25485 140092..140442 + 351 WP_134801370.1 IS66 family insertion sequence element accessory protein TnpB -
C1P70_RS25490 140473..142065 + 1593 WP_001072392.1 IS66 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospC3 / ipaH4.5 / ipaH7.8 / ospD3/senA / ospC1 / senB / ospE2 / ipaH1.4 / ospI / ipgB2 / ospD1 / ospF / ospD2 / ospB / icsP/sopA / nleE / ospC2 / ospG / ipaH9.8 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / icsA/virG / papC 1..216328 216328
- inside IScluster/Tn - ospG / ipaH9.8 117721..161808 44087


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10766.48 Da        Isoelectric Point: 9.9324

>T96285 WP_000421262.1 NZ_CP026829:c138158-137883 [Shigella dysenteriae]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T96285 NZ_CP026829:c138158-137883 [Shigella dysenteriae]
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACAGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAGAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10497.93 Da        Isoelectric Point: 6.2150

>AT96285 WP_001178088.1 NZ_CP026829:c138442-138158 [Shigella dysenteriae]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDHPLPVPR

Download         Length: 285 bp

>AT96285 NZ_CP026829:c138442-138158 [Shigella dysenteriae]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACCATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PVM3

Antitoxin

Source ID Structure
AlphaFold DB Q327A8

References