Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 96250..97025 Replicon plasmid unnamed
Accession NZ_CP026829
Organism Shigella dysenteriae strain ATCC 12037

Toxin (Protein)


Gene name tacT Uniprot ID Q326V1
Locus tag C1P70_RS25170 Protein ID WP_000405248.1
Coordinates 96250..96732 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q7BEG8
Locus tag C1P70_RS25175 Protein ID WP_010921625.1
Coordinates 96723..97025 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P70_RS25140 92336..92488 - 153 Protein_115 integrase core domain-containing protein -
C1P70_RS25145 92544..93236 + 693 WP_000625268.1 type III secretion system effector cysteine methyltransferase OspZ -
C1P70_RS25150 93260..94427 - 1168 Protein_117 IS3 family transposase -
C1P70_RS25155 94501..94839 + 339 Protein_118 integrase core domain-containing protein -
C1P70_RS25160 94882..95393 - 512 Protein_119 transposase -
C1P70_RS25165 95443..96140 + 698 WP_252988899.1 IS1-like element IS1SD family transposase -
C1P70_RS25170 96250..96732 - 483 WP_000405248.1 GNAT family N-acetyltransferase Toxin
C1P70_RS25175 96723..97025 - 303 WP_010921625.1 DUF1778 domain-containing protein Antitoxin
C1P70_RS25185 97297..97569 + 273 WP_000019158.1 hypothetical protein -
C1P70_RS25190 97550..98758 + 1209 WP_134807334.1 IS91-like element ISSbo1 family transposase -
C1P70_RS26035 98930..99058 + 129 Protein_125 IS5/IS1182 family transposase -
C1P70_RS25205 99207..100661 - 1455 WP_134807343.1 type 3 secretion system effector OspC2 -
C1P70_RS25210 101086..101930 + 845 WP_094096542.1 IS5-like element ISSfl7 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospC3 / ipaH4.5 / ipaH7.8 / ospD3/senA / ospC1 / senB / ospE2 / ipaH1.4 / ospI / ipgB2 / ospD1 / ospF / ospD2 / ospB / icsP/sopA / nleE / ospC2 / ospG / ipaH9.8 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / icsA/virG / papC 1..216328 216328
- inside IScluster/Tn - ipgB2 / ospD1 / ospF / ospD2 / ospB / icsP/sopA / nleE / ospC2 63622..108386 44764


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(29-149)

Antitoxin

Vibrio phage ICP1, Orf50

(19-94)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17736.50 Da        Isoelectric Point: 7.0382

>T96282 WP_000405248.1 NZ_CP026829:c96732-96250 [Shigella dysenteriae]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Download         Length: 483 bp

>T96282 NZ_CP026829:c96732-96250 [Shigella dysenteriae]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTGTTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11689.28 Da        Isoelectric Point: 4.9167

>AT96282 WP_010921625.1 NZ_CP026829:c97025-96723 [Shigella dysenteriae]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Download         Length: 303 bp

>AT96282 NZ_CP026829:c97025-96723 [Shigella dysenteriae]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090NWA7

Antitoxin

Source ID Structure
AlphaFold DB Q7BEG8

References