96265

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1876060..1876643 Replicon chromosome
Accession NZ_CP026828
Organism Shigella dysenteriae strain ATCC 12037

Toxin (Protein)


Gene name mazF Uniprot ID Q31XK8
Locus tag C1P70_RS09605 Protein ID WP_000254733.1
Coordinates 1876308..1876643 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag C1P70_RS09600 Protein ID WP_000581937.1
Coordinates 1876060..1876308 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P70_RS09590 (1872399) 1872399..1873700 + 1302 WP_000046825.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
C1P70_RS09595 (1873748) 1873748..1875982 + 2235 WP_000226815.1 GTP pyrophosphokinase -
C1P70_RS09600 (1876060) 1876060..1876308 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
C1P70_RS09605 (1876308) 1876308..1876643 + 336 WP_000254733.1 endoribonuclease MazF Toxin
C1P70_RS09610 (1876714) 1876714..1877505 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
C1P70_RS09615 (1877733) 1877733..1879370 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
C1P70_RS09620 (1879458) 1879458..1880756 + 1299 WP_000036723.1 phosphopyruvate hydratase -
C1P70_RS09625 (1880816) 1880816..1881076 - 261 Protein_1853 YgcG family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1873748..1885782 12034


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12167.15 Da        Isoelectric Point: 8.7218

>T96265 WP_000254733.1 NZ_CP026828:1876308-1876643 [Shigella dysenteriae]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKR
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T96265 NZ_CP026828:1876308-1876643 [Shigella dysenteriae]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGA
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT96265 WP_000581937.1 NZ_CP026828:1876060-1876308 [Shigella dysenteriae]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT96265 NZ_CP026828:1876060-1876308 [Shigella dysenteriae]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q31XK8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LMB4

References