Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4303763..4304260 Replicon chromosome
Accession NZ_CP026827
Organism Shigella dysenteriae strain ATCC 12021

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag C5B75_RS23745 Protein ID WP_001173343.1
Coordinates 4303763..4303936 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag C5B75_RS23750 Protein ID WP_000398619.1
Coordinates 4303964..4304260 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5B75_RS23735 (4300443) 4300443..4301900 + 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -
C5B75_RS23740 (4302137) 4302137..4303644 + 1508 Protein_4337 lysine--tRNA ligase -
C5B75_RS23745 (4303763) 4303763..4303936 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
C5B75_RS23750 (4303964) 4303964..4304260 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
C5B75_RS23755 (4304487) 4304487..4304759 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
C5B75_RS23760 (4304771) 4304771..4305001 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
C5B75_RS23765 (4305182) 4305182..4306813 + 1632 WP_001216486.1 sensor histidine kinase -
C5B75_RS23770 (4306810) 4306810..4307529 + 720 WP_000611296.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T96257 WP_001173343.1 NZ_CP026827:c4303936-4303763 [Shigella dysenteriae]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T96257 NZ_CP026827:c4303936-4303763 [Shigella dysenteriae]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT96257 WP_000398619.1 NZ_CP026827:c4304260-4303964 [Shigella dysenteriae]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT96257 NZ_CP026827:c4304260-4303964 [Shigella dysenteriae]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAGGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References