Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3481549..3482180 Replicon chromosome
Accession NZ_CP026827
Organism Shigella dysenteriae strain ATCC 12021

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag C5B75_RS19605 Protein ID WP_001259385.1
Coordinates 3481905..3482180 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag C5B75_RS19600 Protein ID WP_000593555.1
Coordinates 3481549..3481908 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5B75_RS19575 (3477681) 3477681..3478625 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
C5B75_RS19580 (3478622) 3478622..3479455 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
C5B75_RS19585 (3479455) 3479455..3480219 + 765 WP_001136244.1 nickel import ATP-binding protein NikD -
C5B75_RS19590 (3480216) 3480216..3481022 + 807 WP_000173673.1 nickel import ATP-binding protein NikE -
C5B75_RS19595 (3481028) 3481028..3481429 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
C5B75_RS19600 (3481549) 3481549..3481908 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C5B75_RS19605 (3481905) 3481905..3482180 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
C5B75_RS19610 (3482253) 3482253..3483377 - 1125 WP_001216257.1 ABC transporter permease -
C5B75_RS19615 (3483377) 3483377..3486112 - 2736 WP_119169952.1 ribosome-associated ATPase/putative transporter RbbA -
C5B75_RS19620 (3486109) 3486109..3487176 - 1068 WP_000361498.1 HlyD family efflux transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T96256 WP_001259385.1 NZ_CP026827:c3482180-3481905 [Shigella dysenteriae]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T96256 NZ_CP026827:c3482180-3481905 [Shigella dysenteriae]
ATGAGAACCAAGGTACAGGCTTTGCGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT96256 WP_000593555.1 NZ_CP026827:c3481908-3481549 [Shigella dysenteriae]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT96256 NZ_CP026827:c3481908-3481549 [Shigella dysenteriae]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References