Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2610025..2610643 Replicon chromosome
Accession NZ_CP026827
Organism Shigella dysenteriae strain ATCC 12021

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C5B75_RS15175 Protein ID WP_001291435.1
Coordinates 2610025..2610243 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C5B75_RS15180 Protein ID WP_000344800.1
Coordinates 2610269..2610643 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5B75_RS15140 (2605314) 2605314..2605886 + 573 WP_128875314.1 YbaY family lipoprotein -
C5B75_RS15145 (2605917) 2605917..2606228 - 312 WP_000409911.1 MGMT family protein -
C5B75_RS15155 (2606607) 2606607..2606960 + 354 WP_000878140.1 DUF1428 domain-containing protein -
C5B75_RS15160 (2607002) 2607002..2608552 - 1551 WP_119169836.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C5B75_RS15165 (2608716) 2608716..2609186 - 471 WP_001304825.1 YlaC family protein -
C5B75_RS15170 (2609302) 2609302..2609853 - 552 WP_000102569.1 maltose O-acetyltransferase -
C5B75_RS15175 (2610025) 2610025..2610243 - 219 WP_001291435.1 HHA domain-containing protein Toxin
C5B75_RS15180 (2610269) 2610269..2610643 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C5B75_RS15185 (2611189) 2611189..2614338 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
C5B75_RS15190 (2614361) 2614361..2615554 - 1194 WP_097312698.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T96250 WP_001291435.1 NZ_CP026827:c2610243-2610025 [Shigella dysenteriae]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T96250 NZ_CP026827:c2610243-2610025 [Shigella dysenteriae]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT96250 WP_000344800.1 NZ_CP026827:c2610643-2610269 [Shigella dysenteriae]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT96250 NZ_CP026827:c2610643-2610269 [Shigella dysenteriae]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References