Detailed information of TA system TA096238

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 437598..438223
• Replicon: chromosome
• Accession: NZ_CP026827
• Organism: Shigella dysenteriae strain ATCC 12021

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C5B75_RS03725
• Protein ID: WP_000911330.1
• Coordinates: 437825..438223 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: C5B75_RS03720
• Protein ID: WP_000450524.1
• Coordinates: 437598..437825 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5B75_RS03695 (433401)	433401..433871	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
C5B75_RS03700 (433871)	433871..434443	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
C5B75_RS03705 (434589)	434589..435467	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
C5B75_RS03710 (435484)	435484..436518	+	1035	WP_128875018.1	outer membrane protein assembly factor BamC	-
C5B75_RS03715 (436731)	436731..437444	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
C5B75_RS03720 (437598)	437598..437825	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
C5B75_RS03725 (437825)	437825..438223	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
C5B75_RS03730 (438370)	438370..439233	+	864	WP_072665489.1	neutral zinc metallopeptidase	-
C5B75_RS03735 (439248)	439248..441263	+	2016	WP_047084717.1	tRNA cytosine(34) acetyltransferase TmcA	-
C5B75_RS03740 (441337)	441337..442035	+	699	WP_000679812.1	esterase	-
C5B75_RS03745 (442145)	442145..442345	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T96238 WP_000911330.1 NZ_CP026827:437825-438223 [Shigella dysenteriae]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T96238 NZ_CP026827:437825-438223 [Shigella dysenteriae]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT96238 WP_000450524.1 NZ_CP026827:437598-437825 [Shigella dysenteriae]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT96238 NZ_CP026827:437598-437825 [Shigella dysenteriae]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33