Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 164274..164857 Replicon chromosome
Accession NZ_CP026827
Organism Shigella dysenteriae strain ATCC 12021

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag C5B75_RS02330 Protein ID WP_000254739.1
Coordinates 164522..164857 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag C5B75_RS02325 Protein ID WP_000581937.1
Coordinates 164274..164522 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5B75_RS02315 (160613) 160613..161914 + 1302 WP_042110359.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
C5B75_RS02320 (161962) 161962..164196 + 2235 WP_000226815.1 GTP pyrophosphokinase -
C5B75_RS02325 (164274) 164274..164522 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
C5B75_RS02330 (164522) 164522..164857 + 336 WP_000254739.1 endoribonuclease MazF Toxin
C5B75_RS02335 (164928) 164928..165719 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
C5B75_RS02340 (165947) 165947..167584 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
C5B75_RS02345 (167672) 167672..168970 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12126.09 Da        Isoelectric Point: 8.2618

>T96236 WP_000254739.1 NZ_CP026827:164522-164857 [Shigella dysenteriae]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVVPEELQLIKAKINVLIG

Download         Length: 336 bp

>T96236 NZ_CP026827:164522-164857 [Shigella dysenteriae]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGTCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT96236 WP_000581937.1 NZ_CP026827:164274-164522 [Shigella dysenteriae]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT96236 NZ_CP026827:164274-164522 [Shigella dysenteriae]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References