Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 184014..184771 Replicon plasmid unnamed
Accession NZ_CP026826
Organism Shigella dysenteriae strain ATCC 12021

Toxin (Protein)


Gene name tacT Uniprot ID Q31SN7
Locus tag C5B75_RS01290 Protein ID WP_000501969.1
Coordinates 184014..184499 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q31SN8
Locus tag C5B75_RS01295 Protein ID WP_011114751.1
Coordinates 184487..184771 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5B75_RS01250 179061..179216 - 156 Protein_227 IS256 family transposase -
C5B75_RS01260 179323..179595 + 273 WP_000019158.1 hypothetical protein -
C5B75_RS01265 179576..180784 + 1209 WP_128874952.1 IS91 family transposase -
C5B75_RS01270 180854..182041 + 1188 WP_181876988.1 hypothetical protein -
C5B75_RS01275 182111..182251 + 141 Protein_231 IS3 family transposase -
C5B75_RS01285 183509..183841 + 333 Protein_233 IS4/IS5 family transposase -
C5B75_RS01290 184014..184499 - 486 WP_000501969.1 GNAT family N-acetyltransferase Toxin
C5B75_RS01295 184487..184771 - 285 WP_011114751.1 DUF1778 domain-containing protein Antitoxin
C5B75_RS25330 185680..185844 + 165 WP_001346193.1 hypothetical protein -
C5B75_RS01320 186098..187619 - 1522 Protein_237 IS66 family transposase -
C5B75_RS01325 187639..187821 - 183 Protein_238 IS66 family insertion sequence element accessory protein TnpB -
C5B75_RS01335 188096..189115 - 1020 WP_128874966.1 IS110-like element ISEc11 family transposase -
C5B75_RS01340 189264..189428 - 165 Protein_240 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ipaC / ipaC / ipaB / ipaB / ipaB / ipgC / ipgC / ipgB1 / icsB / icsB / ipgD / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ospC2 / nleE / icsP/sopA / ospB / ospC4 / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ospC1 / ospD3/senA / ipaH7.8 / ipaH4.5 / ipaH1.4 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / mxiA / mxiA / spa15 / spa15 / spa47 / spa47 / spa13 1..224352 224352
- inside IScluster/Tn - ospE2 / ospC1 / ospD3/senA / ipaH7.8 / ipaH4.5 / ipaH1.4 / ospC3 144756..196035 51279


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(30-151)

Antitoxin

Vibrio phage ICP1, Orf50

(12-88)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17712.54 Da        Isoelectric Point: 10.1083

>T96235 WP_000501969.1 NZ_CP026826:c184499-184014 [Shigella dysenteriae]
MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Download         Length: 486 bp

>T96235 NZ_CP026826:c184499-184014 [Shigella dysenteriae]
ATGGGATGTGTAACTGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCGGTGAAGCTGTACTTGA
TGACTGGTTAAAGCAGAAGGGGCTAAAAAATCAGGCAATCGGAGCCACCCGGACTTTTGTTGTATGCAGAAAAGGTACGC
AACAAATAGTCGGTTTTTACTCACTGGCCACTGGTAGTGTCAATCATACAGAAGCTACAGGTAACCTTCGGCGCAATATG
CCAGACCCCATTCCTGTAATTATACTTGCACGCCTGGCTGTAGATGTGTCATTTCGAGGAAAAGGGCTTGGCGCGGATTT
ACTTCATGATGCAGTTCGTCGTTGTTACAGGGTTGCCGAAAATATCGGTGTGCGTGCAATTATGGTTCATGCGCTTACGG
AAAACGCAAAACAGTTTTATATTCATCATGGTTTTAAGCCATCAAAAACTCAGGTGCAAACATTGTTTCTTAAACTCCCT
CAATAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10871.52 Da        Isoelectric Point: 4.6812

>AT96235 WP_011114751.1 NZ_CP026826:c184771-184487 [Shigella dysenteriae]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Download         Length: 285 bp

>AT96235 NZ_CP026826:c184771-184487 [Shigella dysenteriae]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAGCTCAATCTCAGAGCAAGAGAATCACAGCGGATACTCATTGATGC
TGCTGCAGAAATTCTTCATAAGTCGCGTACAGATTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATGTTATTCTCG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATTGAAATGCTTGATGCTCCGGTTGCAGATGATCCT
GCCATCGAAAAATTGCTGGCAAGGAAACCTCAATGGGATGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q31SN7

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CUJ1

References