Detailed information of TA system TA096234

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 105390..106165
• Replicon: plasmid unnamed
• Accession: NZ_CP026826
• Organism: Shigella dysenteriae strain ATCC 12021

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: C5B75_RS00745
• Protein ID: WP_128874955.1
• Coordinates: 105683..106165 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: Q7BEG8
• Locus tag: C5B75_RS00740
• Protein ID: WP_010921625.1
• Coordinates: 105390..105692 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5B75_RS00700	101020..102474	+	1455	WP_128874954.1	type 3 secretion system effector OspC2	-
C5B75_RS25065	102623..102751	-	129	Protein_125	IS5/IS1182 family transposase	-
C5B75_RS25615	102870..103037	-	168	Protein_126	IS91 family transposase	-
C5B75_RS00720	103018..103290	-	273	WP_000019158.1	hypothetical protein	-
C5B75_RS00730	103558..104745	-	1188	WP_128874976.1	IS91 family transposase	-
C5B75_RS00735	104745..105110	-	366	WP_000124126.1	hypothetical protein	-
C5B75_RS00740	105390..105692	+	303	WP_010921625.1	DUF1778 domain-containing protein	Antitoxin
C5B75_RS00745	105683..106165	+	483	WP_128874955.1	GNAT family N-acetyltransferase	Toxin
C5B75_RS00750	106233..106756	+	524	Protein_132	transposase	-
C5B75_RS00755	106799..107137	-	339	Protein_133	integrase core domain-containing protein	-
C5B75_RS00760	107211..108379	+	1169	WP_086020965.1	IS3-like element IS911 family transposase	-
C5B75_RS00765	108439..109103	-	665	Protein_135	type III secretion system effector cysteine methyltransferase OspZ	-
C5B75_RS00770	109159..109311	+	153	Protein_136	integrase core domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ipaC / ipaC / ipaB / ipaB / ipaB / ipgC / ipgC / ipgB1 / icsB / icsB / ipgD / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ospC2 / nleE / icsP/sopA / ospB / ospC4 / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ospC1 / ospD3/senA / ipaH7.8 / ipaH4.5 / ipaH1.4 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / mxiA / mxiA / spa15 / spa15 / spa47 / spa47 / spa13	1..224352	224352
	-	inside	IScluster/Tn	-	ospC2 / nleE / icsP/sopA / ospB / ospC4 / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2	95002..138900	43898

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (29-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17718.46 Da
• Isoelectric Point: 7.0382

>T96234 WP_128874955.1 NZ_CP026826:105683-106165 [Shigella dysenteriae]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVIVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Nucleotide

• Length: 483 bp

>T96234 NZ_CP026826:105683-106165 [Shigella dysenteriae]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTGTTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATAGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11689.28 Da
• Isoelectric Point: 4.9167

>AT96234 WP_010921625.1 NZ_CP026826:105390-105692 [Shigella dysenteriae]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Nucleotide

• Length: 303 bp

>AT96234 NZ_CP026826:105390-105692 [Shigella dysenteriae]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAATATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Structures

Antitoxin

• AlphaFold DB: Q7BEG8