Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 144303..144928 Replicon plasmid unnamed2
Accession NZ_CP026820
Organism Shigella dysenteriae strain 96-265

Toxin (Protein)


Gene name vapC Uniprot ID Q6XVU6
Locus tag C1P77_RS26405 Protein ID WP_000911318.1
Coordinates 144303..144701 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q31SG2
Locus tag C1P77_RS26410 Protein ID WP_000450530.1
Coordinates 144701..144928 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P77_RS26380 139384..140328 + 945 WP_005027201.1 lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase -
C1P77_RS26385 140482..141153 - 672 Protein_172 IS630 family transposase -
C1P77_RS26400 142114..144294 + 2181 WP_069661558.1 type IV conjugative transfer system coupling protein TraD -
C1P77_RS26405 144303..144701 - 399 WP_000911318.1 type II toxin-antitoxin system VapC family toxin Toxin
C1P77_RS26410 144701..144928 - 228 WP_000450530.1 toxin-antitoxin system antitoxin VapB Antitoxin

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospI / ospE2 / ipaH1.4 / ospC1 / ospD3/senA / ipaH7.8 / ipaH4.5 / ospC3 / ipaJ / ipaA / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / virA / icsA/virG / papC / ipaH9.8 / ospG / ospC2 / nleE / icsP/sopA / icsP/sopA / ospB / ospC4 / ospD2 / ospF / ospD1 / ipgB2 1..195489 195489
- inside IScluster/Tn - ipaH9.8 / ospG 108979..141672 32693


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14845.09 Da        Isoelectric Point: 8.5264

>T96231 WP_000911318.1 NZ_CP026820:c144701-144303 [Shigella dysenteriae]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRTEDWS

Download         Length: 399 bp

>T96231 NZ_CP026820:c144701-144303 [Shigella dysenteriae]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCTGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAACTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8535.59 Da        Isoelectric Point: 4.7513

>AT96231 WP_000450530.1 NZ_CP026820:c144928-144701 [Shigella dysenteriae]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHSVSADFMDNREQPGMQERESF

Download         Length: 228 bp

>AT96231 NZ_CP026820:c144928-144701 [Shigella dysenteriae]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6SD6

Antitoxin

Source ID Structure
AlphaFold DB Q31SG2

References