Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3088563..3089217 Replicon chromosome
Accession NZ_CP026819
Organism Shigella dysenteriae strain 96-265

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag C1P77_RS17095 Protein ID WP_000244777.1
Coordinates 3088810..3089217 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C1P77_RS17090 Protein ID WP_000354046.1
Coordinates 3088563..3088829 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P77_RS17065 (3083851) 3083851..3084594 + 744 WP_000951947.1 SDR family oxidoreductase -
C1P77_RS17070 (3084651) 3084651..3086084 - 1434 WP_004986559.1 6-phospho-beta-glucosidase BglA -
C1P77_RS17075 (3086129) 3086129..3086440 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
C1P77_RS17080 (3086604) 3086604..3087263 + 660 WP_000250274.1 hemolysin III family protein -
C1P77_RS17085 (3087340) 3087340..3088320 - 981 WP_000886098.1 tRNA-modifying protein YgfZ -
C1P77_RS17090 (3088563) 3088563..3088829 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C1P77_RS17095 (3088810) 3088810..3089217 + 408 WP_000244777.1 protein YgfX Toxin
C1P77_RS17100 (3089257) 3089257..3089778 - 522 WP_001055874.1 flavodoxin FldB -
C1P77_RS17105 (3089890) 3089890..3090786 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C1P77_RS17110 (3090811) 3090811..3091521 + 711 WP_000715205.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C1P77_RS17115 (3091527) 3091527..3093260 + 1734 WP_000813209.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T96222 WP_000244777.1 NZ_CP026819:3088810-3089217 [Shigella dysenteriae]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T96222 NZ_CP026819:3088810-3089217 [Shigella dysenteriae]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGATTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT96222 WP_000354046.1 NZ_CP026819:3088563-3088829 [Shigella dysenteriae]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT96222 NZ_CP026819:3088563-3088829 [Shigella dysenteriae]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References