Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2619525..2620108 Replicon chromosome
Accession NZ_CP026819
Organism Shigella dysenteriae strain 96-265

Toxin (Protein)


Gene name mazF Uniprot ID Q31XK8
Locus tag C1P77_RS14705 Protein ID WP_000254733.1
Coordinates 2619525..2619860 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag C1P77_RS14710 Protein ID WP_000581937.1
Coordinates 2619860..2620108 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P77_RS14685 (2615031) 2615031..2615357 + 327 Protein_2738 YgcG family protein -
C1P77_RS14690 (2615417) 2615417..2616715 - 1299 WP_000036723.1 phosphopyruvate hydratase -
C1P77_RS14695 (2616803) 2616803..2618440 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
C1P77_RS14700 (2618663) 2618663..2619454 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
C1P77_RS14705 (2619525) 2619525..2619860 - 336 WP_000254733.1 endoribonuclease MazF Toxin
C1P77_RS14710 (2619860) 2619860..2620108 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
C1P77_RS14715 (2620186) 2620186..2622420 - 2235 WP_000226815.1 GTP pyrophosphokinase -
C1P77_RS14720 (2622468) 2622468..2623769 - 1302 WP_000046825.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12167.15 Da        Isoelectric Point: 8.7218

>T96219 WP_000254733.1 NZ_CP026819:c2619860-2619525 [Shigella dysenteriae]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKR
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T96219 NZ_CP026819:c2619860-2619525 [Shigella dysenteriae]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGA
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT96219 WP_000581937.1 NZ_CP026819:c2620108-2619860 [Shigella dysenteriae]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT96219 NZ_CP026819:c2620108-2619860 [Shigella dysenteriae]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q31XK8

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References