Detailed information of TA system TA096203

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 41470..42071
• Replicon: plasmid unnamed1
• Accession: NZ_CP026818
• Organism: Shigella dysenteriae strain 96-265

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A6N8QT96
• Locus tag: C1P77_RS00290
• Protein ID: WP_032220967.1
• Coordinates: 41691..42071 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: C1P77_RS00285
• Protein ID: WP_001190712.1
• Coordinates: 41470..41691 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P77_RS00235	36806..37558	+	753	WP_134800768.1	ead/Ea22-like family protein	-
C1P77_RS00240	37560..38177	+	618	WP_134805652.1	hypothetical protein	-
C1P77_RS00245	38174..38728	+	555	WP_134800769.1	DUF551 domain-containing protein	-
C1P77_RS00250	38721..39002	+	282	WP_134800770.1	ASCH domain-containing protein	-
C1P77_RS00255	39026..39319	+	294	WP_134800771.1	hypothetical protein	-
C1P77_RS26835	39319..39477	+	159	WP_284512731.1	hypothetical protein	-
C1P77_RS00260	39560..39934	+	375	WP_134800772.1	hypothetical protein	-
C1P77_RS00265	39916..40569	+	654	WP_134810118.1	hypothetical protein	-
C1P77_RS00275	40633..40884	+	252	WP_001283837.1	DNA polymerase III subunit theta	-
C1P77_RS00280	41008..41397	+	390	WP_097295905.1	S24 family peptidase	-
C1P77_RS00285	41470..41691	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C1P77_RS00290	41691..42071	+	381	WP_032220967.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
C1P77_RS00295	42155..42961	-	807	WP_134800773.1	hypothetical protein	-
C1P77_RS00300	42968..43408	-	441	WP_134805665.1	DUF2829 domain-containing protein	-
C1P77_RS00305	43423..43917	-	495	WP_110021427.1	dUTP diphosphatase	-
C1P77_RS00310	43910..44401	-	492	WP_134800774.1	hypothetical protein	-
C1P77_RS00315	44398..44742	-	345	WP_004010537.1	hypothetical protein	-
C1P77_RS00320	44815..45843	-	1029	WP_134805664.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..80213	80213

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13645.56 Da
• Isoelectric Point: 5.6406

>T96203 WP_032220967.1 NZ_CP026818:41691-42071 [Shigella dysenteriae]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRELFGPNI

Nucleotide

• Length: 381 bp

>T96203 NZ_CP026818:41691-42071 [Shigella dysenteriae]
ATGAGGCATATATCACCGGAAGAACTGATAGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACGGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGTATACAGGTATATGATTCTCCCGTGCTGGTGGAACTTGCGGTGGGGGCCGCAACTGG
TGAAATCCCCGTATCTTCAGTAGCGGAAAAACTACGTGAATTATTTGGTCCCAATATTTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT96203 WP_001190712.1 NZ_CP026818:41470-41691 [Shigella dysenteriae]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT96203 NZ_CP026818:41470-41691 [Shigella dysenteriae]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A6N8QT96

Antitoxin

• AlphaFold DB: A0A829CJB6