Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 157077..157702 Replicon plasmid unnamed2
Accession NZ_CP026817
Organism Shigella dysenteriae strain 93-119

Toxin (Protein)


Gene name vapC Uniprot ID Q6XVU6
Locus tag C1P76_RS26895 Protein ID WP_000911318.1
Coordinates 157304..157702 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q31SG2
Locus tag C1P76_RS26890 Protein ID WP_000450530.1
Coordinates 157077..157304 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P76_RS26890 157077..157304 + 228 WP_000450530.1 toxin-antitoxin system antitoxin VapB Antitoxin
C1P76_RS26895 157304..157702 + 399 WP_000911318.1 type II toxin-antitoxin system VapC family toxin Toxin
C1P76_RS26900 157711..159890 - 2180 Protein_192 type IV conjugative transfer system coupling protein TraD -
C1P76_RS26905 160138..160835 + 698 WP_094096780.1 IS1 family transposase -
C1P76_RS26910 160851..161522 + 672 Protein_194 IS630 family transposase -
C1P76_RS26915 161676..162620 - 945 WP_005027201.1 lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaA / ipaJ / ospC3 / ipaH4.5 / ipaH7.8 / ospD3/senA / ospC1 / senB / ipaH1.4 / ospE2 / ospI / ipgB2 / ospD1 / ospF / ospD2 / ospD2 / ospC4 / ospB / icsP/sopA / nleE / ospC2 / ospG / ipaH9.8 / papC / icsA/virG / virA / spa40 / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA 1..226441 226441
- inside IScluster/Tn - ospG / ipaH9.8 160332..196012 35680


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14845.09 Da        Isoelectric Point: 8.5264

>T96200 WP_000911318.1 NZ_CP026817:157304-157702 [Shigella dysenteriae]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRTEDWS

Download         Length: 399 bp

>T96200 NZ_CP026817:157304-157702 [Shigella dysenteriae]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCTGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAACTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8535.59 Da        Isoelectric Point: 4.7513

>AT96200 WP_000450530.1 NZ_CP026817:157077-157304 [Shigella dysenteriae]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHSVSADFMDNREQPGMQERESF

Download         Length: 228 bp

>AT96200 NZ_CP026817:157077-157304 [Shigella dysenteriae]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6SD6

Antitoxin

Source ID Structure
AlphaFold DB Q31SG2

References