Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 135828..136603 Replicon plasmid unnamed2
Accession NZ_CP026817
Organism Shigella dysenteriae strain 93-119

Toxin (Protein)


Gene name tacT Uniprot ID Q326V1
Locus tag C1P76_RS26740 Protein ID WP_000405248.1
Coordinates 135828..136310 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q7BEG8
Locus tag C1P76_RS26745 Protein ID WP_010921625.1
Coordinates 136301..136603 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P76_RS28240 132272..132409 + 138 WP_005026553.1 hypothetical protein -
C1P76_RS26715 132691..132843 - 153 Protein_160 IS3 family transposase -
C1P76_RS26720 132899..133591 + 693 WP_000625268.1 type III secretion system effector cysteine methyltransferase OspZ -
C1P76_RS26725 133615..134782 - 1168 Protein_162 IS3 family transposase -
C1P76_RS26730 134856..135194 + 339 Protein_163 integrase core domain-containing protein -
C1P76_RS26735 135237..135760 - 524 Protein_164 transposase -
C1P76_RS26740 135828..136310 - 483 WP_000405248.1 GNAT family N-acetyltransferase Toxin
C1P76_RS26745 136301..136603 - 303 WP_010921625.1 DUF1778 domain-containing protein Antitoxin
C1P76_RS26755 136875..137147 + 273 WP_000019158.1 hypothetical protein -
C1P76_RS26760 137128..138336 + 1209 WP_046891828.1 IS91-like element ISSbo1 family transposase -
C1P76_RS27440 138508..138636 + 129 Protein_169 IS5/IS1182 family transposase -
C1P76_RS26775 138785..140239 - 1455 WP_000701106.1 type 3 secretion system effector OspC2 -
C1P76_RS26780 140664..141508 + 845 WP_094096542.1 IS5-like element ISSfl7 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaA / ipaJ / ospC3 / ipaH4.5 / ipaH7.8 / ospD3/senA / ospC1 / senB / ipaH1.4 / ospE2 / ospI / ipgB2 / ospD1 / ospF / ospD2 / ospD2 / ospC4 / ospB / icsP/sopA / nleE / ospC2 / ospG / ipaH9.8 / papC / icsA/virG / virA / spa40 / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA 1..226441 226441
- inside IScluster/Tn - ipgB2 / ospD1 / ospF / ospD2 / ospD2 / ospC4 / ospB / icsP/sopA / nleE / ospC2 103982..145283 41301


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(29-149)

Antitoxin

Vibrio phage ICP1, Orf50

(19-94)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17736.50 Da        Isoelectric Point: 7.0382

>T96199 WP_000405248.1 NZ_CP026817:c136310-135828 [Shigella dysenteriae]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Download         Length: 483 bp

>T96199 NZ_CP026817:c136310-135828 [Shigella dysenteriae]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTGTTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11689.28 Da        Isoelectric Point: 4.9167

>AT96199 WP_010921625.1 NZ_CP026817:c136603-136301 [Shigella dysenteriae]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Download         Length: 303 bp

>AT96199 NZ_CP026817:c136603-136301 [Shigella dysenteriae]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090NWA7

Antitoxin

Source ID Structure
AlphaFold DB Q7BEG8

References