Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 85622..86379 Replicon plasmid unnamed
Accession NZ_CP026813
Organism Shigella boydii strain 83-578

Toxin (Protein)


Gene name tacT Uniprot ID Q31SN7
Locus tag C1P60_RS00615 Protein ID WP_000501969.1
Coordinates 85622..86107 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q31SN8
Locus tag C1P60_RS00620 Protein ID WP_011114751.1
Coordinates 86095..86379 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P60_RS00585 80757..81966 - 1210 Protein_100 IS3 family transposase -
C1P60_RS25450 82020..82091 + 72 Protein_101 IS66 family insertion sequence element accessory protein TnpB -
C1P60_RS00595 82088..82438 + 351 Protein_102 IS66 family insertion sequence element accessory protein TnpB -
C1P60_RS00600 82469..84061 + 1593 WP_001072392.1 IS66 family transposase -
C1P60_RS00610 84354..85481 - 1128 WP_001063816.1 IS110-like element ISSso6 family transposase -
C1P60_RS00615 85622..86107 - 486 WP_000501969.1 GNAT family N-acetyltransferase Toxin
C1P60_RS00620 86095..86379 - 285 WP_011114751.1 DUF1778 domain-containing protein Antitoxin
C1P60_RS00635 87052..88220 + 1169 WP_134802537.1 IS3 family transposase -
C1P60_RS00640 88230..88356 - 127 Protein_108 IS110 family transposase -
C1P60_RS00655 89920..90189 - 270 Protein_110 transposase -
C1P60_RS25215 90304..90942 - 639 WP_000607008.1 type III secretion system effector OspI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ipaH9.8 / ospG / ospC2 / nleE / icsP/sopA / ospB / ospC4 / ospD2 / ospF / ospD1 / ipgB2 / ospI / ipaH1.4 / ospE2 1..122029 122029
- inside IScluster/Tn - ospI / ipaH1.4 / ospE2 80757..121983 41226


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(30-151)

Antitoxin

Vibrio phage ICP1, Orf50

(12-88)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17712.54 Da        Isoelectric Point: 10.1083

>T96153 WP_000501969.1 NZ_CP026813:c86107-85622 [Shigella boydii]
MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Download         Length: 486 bp

>T96153 NZ_CP026813:c86107-85622 [Shigella boydii]
ATGGGATGTGTAACTGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCGGTGAAGCTGTACTTGA
TGACTGGTTAAAGCAGAAGGGGCTAAAAAATCAGGCAATCGGAGCCACCCGGACTTTTGTTGTATGCAGAAAAGGTACGC
AACAAATAGTCGGTTTTTACTCACTGGCCACTGGTAGTGTCAATCATACAGAAGCTACAGGTAACCTTCGGCGCAATATG
CCAGACCCCATTCCTGTAATTATACTTGCACGCCTGGCTGTAGATGTGTCATTTCGAGGAAAAGGGCTTGGCGCGGATTT
ACTTCATGATGCAGTTCGTCGTTGTTACAGGGTTGCCGAAAATATCGGTGTGCGTGCAATTATGGTTCATGCGCTTACGG
AAAACGCAAAACAGTTTTATATTCATCATGGTTTTAAGCCATCAAAAACTCAGGTGCAAACATTGTTTCTTAAACTCCCT
CAATAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10871.52 Da        Isoelectric Point: 4.6812

>AT96153 WP_011114751.1 NZ_CP026813:c86379-86095 [Shigella boydii]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Download         Length: 285 bp

>AT96153 NZ_CP026813:c86379-86095 [Shigella boydii]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAGCTCAATCTCAGAGCAAGAGAATCACAGCGGATACTCATTGATGC
TGCTGCAGAAATTCTTCATAAGTCGCGTACAGATTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATGTTATTCTCG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATTGAAATGCTTGATGCTCCGGTTGCAGATGATCCT
GCCATCGAAAAATTGCTGGCAAGGAAACCTCAATGGGATGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q31SN7

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CUJ1

References