Detailed information of TA system TA096153

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 85622..86379
• Replicon: plasmid unnamed
• Accession: NZ_CP026813
• Organism: Shigella boydii strain 83-578

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q31SN7
• Locus tag: C1P60_RS00615
• Protein ID: WP_000501969.1
• Coordinates: 85622..86107 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: Q31SN8
• Locus tag: C1P60_RS00620
• Protein ID: WP_011114751.1
• Coordinates: 86095..86379 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P60_RS00585	80757..81966	-	1210	Protein_100	IS3 family transposase	-
C1P60_RS25450	82020..82091	+	72	Protein_101	IS66 family insertion sequence element accessory protein TnpB	-
C1P60_RS00595	82088..82438	+	351	Protein_102	IS66 family insertion sequence element accessory protein TnpB	-
C1P60_RS00600	82469..84061	+	1593	WP_001072392.1	IS66 family transposase	-
C1P60_RS00610	84354..85481	-	1128	WP_001063816.1	IS110-like element ISSso6 family transposase	-
C1P60_RS00615	85622..86107	-	486	WP_000501969.1	GNAT family N-acetyltransferase	Toxin
C1P60_RS00620	86095..86379	-	285	WP_011114751.1	DUF1778 domain-containing protein	Antitoxin
C1P60_RS00635	87052..88220	+	1169	WP_134802537.1	IS3 family transposase	-
C1P60_RS00640	88230..88356	-	127	Protein_108	IS110 family transposase	-
C1P60_RS00655	89920..90189	-	270	Protein_110	transposase	-
C1P60_RS25215	90304..90942	-	639	WP_000607008.1	type III secretion system effector OspI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ipaH9.8 / ospG / ospC2 / nleE / icsP/sopA / ospB / ospC4 / ospD2 / ospF / ospD1 / ipgB2 / ospI / ipaH1.4 / ospE2	1..122029	122029
	-	inside	IScluster/Tn	-	ospI / ipaH1.4 / ospE2	80757..121983	41226

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (30-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (12-88)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17712.54 Da
• Isoelectric Point: 10.1083

>T96153 WP_000501969.1 NZ_CP026813:c86107-85622 [Shigella boydii]
MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T96153 NZ_CP026813:c86107-85622 [Shigella boydii]
ATGGGATGTGTAACTGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCGGTGAAGCTGTACTTGA
TGACTGGTTAAAGCAGAAGGGGCTAAAAAATCAGGCAATCGGAGCCACCCGGACTTTTGTTGTATGCAGAAAAGGTACGC
AACAAATAGTCGGTTTTTACTCACTGGCCACTGGTAGTGTCAATCATACAGAAGCTACAGGTAACCTTCGGCGCAATATG
CCAGACCCCATTCCTGTAATTATACTTGCACGCCTGGCTGTAGATGTGTCATTTCGAGGAAAAGGGCTTGGCGCGGATTT
ACTTCATGATGCAGTTCGTCGTTGTTACAGGGTTGCCGAAAATATCGGTGTGCGTGCAATTATGGTTCATGCGCTTACGG
AAAACGCAAAACAGTTTTATATTCATCATGGTTTTAAGCCATCAAAAACTCAGGTGCAAACATTGTTTCTTAAACTCCCT
CAATAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10871.52 Da
• Isoelectric Point: 4.6812

>AT96153 WP_011114751.1 NZ_CP026813:c86379-86095 [Shigella boydii]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Nucleotide

• Length: 285 bp

>AT96153 NZ_CP026813:c86379-86095 [Shigella boydii]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAGCTCAATCTCAGAGCAAGAGAATCACAGCGGATACTCATTGATGC
TGCTGCAGAAATTCTTCATAAGTCGCGTACAGATTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATGTTATTCTCG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATTGAAATGCTTGATGCTCCGGTTGCAGATGATCCT
GCCATCGAAAAATTGCTGGCAAGGAAACCTCAATGGGATGTGTAA

Structures

Toxin

• AlphaFold DB: Q31SN7

Antitoxin

• AlphaFold DB: A0A4V1CUJ1