Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 18736..19361 Replicon plasmid unnamed
Accession NZ_CP026813
Organism Shigella boydii strain 83-578

Toxin (Protein)


Gene name vapC Uniprot ID A0A2S8DLI0
Locus tag C1P60_RS00130 Protein ID WP_000911319.1
Coordinates 18736..19134 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q31SG2
Locus tag C1P60_RS00135 Protein ID WP_000450530.1
Coordinates 19134..19361 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P60_RS00105 13808..14752 + 945 WP_012421740.1 lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase -
C1P60_RS00110 14907..15578 - 672 Protein_20 IS630 family transposase -
C1P60_RS00115 15594..16291 - 698 Protein_21 IS1 family transposase -
C1P60_RS00125 16539..18727 + 2189 Protein_22 type IV conjugative transfer system coupling protein TraD -
C1P60_RS00130 18736..19134 - 399 WP_000911319.1 tRNA(fMet)-specific endonuclease VapC Toxin
C1P60_RS00135 19134..19361 - 228 WP_000450530.1 toxin-antitoxin system antitoxin VapB Antitoxin
C1P60_RS00140 19565..19858 + 294 WP_171548087.1 relaxase domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ipaH9.8 / ospG / ospC2 / nleE / icsP/sopA / ospB / ospC4 / ospD2 / ospF / ospD1 / ipgB2 / ospI / ipaH1.4 / ospE2 1..122029 122029
- inside IScluster/Tn - ipaH9.8 / ospG 742..16097 15355


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14887.17 Da        Isoelectric Point: 8.5264

>T96151 WP_000911319.1 NZ_CP026813:c19134-18736 [Shigella boydii]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
VQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRTEDWS

Download         Length: 399 bp

>T96151 NZ_CP026813:c19134-18736 [Shigella boydii]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCTGCAGCCACACACACC
GTCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAACTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8535.59 Da        Isoelectric Point: 4.7513

>AT96151 WP_000450530.1 NZ_CP026813:c19361-19134 [Shigella boydii]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHSVSADFMDNREQPGMQERESF

Download         Length: 228 bp

>AT96151 NZ_CP026813:c19361-19134 [Shigella boydii]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S8DLI0

Antitoxin

Source ID Structure
AlphaFold DB Q31SG2

References