Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5688..6213 Replicon plasmid unnamed
Accession NZ_CP026813
Organism Shigella boydii strain 83-578

Toxin (Protein)


Gene name ccdB Uniprot ID Q326Z8
Locus tag C1P60_RS00055 Protein ID WP_001159860.1
Coordinates 5908..6213 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q7BEK0
Locus tag C1P60_RS00050 Protein ID WP_000813626.1
Coordinates 5688..5906 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P60_RS00015 1429..2178 - 750 WP_000828661.1 phosphatase PAP2 family protein -
C1P60_RS00025 2541..2756 + 216 Protein_3 DDE-type integrase/transposase/recombinase -
C1P60_RS25435 3039..3482 + 444 WP_201797260.1 hypothetical protein -
C1P60_RS00035 3534..4747 + 1214 WP_167692677.1 IS3 family transposase -
C1P60_RS00040 4823..5095 - 273 WP_012421738.1 hypothetical protein -
C1P60_RS00050 5688..5906 + 219 WP_000813626.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C1P60_RS00055 5908..6213 + 306 WP_001159860.1 type II toxin-antitoxin system toxin CcdB Toxin
C1P60_RS00060 6249..6344 + 96 Protein_9 transposase domain-containing protein -
C1P60_RS00065 6606..8243 + 1638 WP_012421769.1 T3SS effector E3 ubiquitin-protein ligase IpaH9.8 -
C1P60_RS00070 8451..8720 + 270 WP_000079956.1 hypothetical protein -
C1P60_RS25440 8717..8943 + 227 Protein_12 type II toxin-antitoxin system RelE/ParE family toxin -
C1P60_RS00080 8972..9457 + 486 WP_039058926.1 protein kinase -
C1P60_RS00085 9472..10169 - 698 Protein_14 IS1 family transposase -
C1P60_RS25195 10339..10491 + 153 WP_171765824.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ipaH9.8 / ospG / ospC2 / nleE / icsP/sopA / ospB / ospC4 / ospD2 / ospF / ospD1 / ipgB2 / ospI / ipaH1.4 / ospE2 1..122029 122029
- inside IScluster/Tn - ipaH9.8 / ospG 742..16097 15355


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11736.59 Da        Isoelectric Point: 6.4674

>T96150 WP_001159860.1 NZ_CP026813:5908-6213 [Shigella boydii]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T96150 NZ_CP026813:5908-6213 [Shigella boydii]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTTTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGATCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8333.36 Da        Isoelectric Point: 6.2838

>AT96150 WP_000813626.1 NZ_CP026813:5688-5906 [Shigella boydii]
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT96150 NZ_CP026813:5688-5906 [Shigella boydii]
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTN3

Antitoxin

Source ID Structure
AlphaFold DB Q7BEK0

References