Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 154872..155497 Replicon plasmid unnamed
Accession NZ_CP026812
Organism Shigella flexneri strain 64-5500

Toxin (Protein)


Gene name vapC Uniprot ID Q6XVU6
Locus tag C1P83_RS25330 Protein ID WP_000911318.1
Coordinates 154872..155270 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q31SG2
Locus tag C1P83_RS25335 Protein ID WP_000450530.1
Coordinates 155270..155497 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P83_RS25295 150014..150945 + 932 Protein_184 IS3 family transposase -
C1P83_RS25300 150979..152571 - 1593 WP_001072385.1 IS66 family transposase -
C1P83_RS25305 152602..152952 - 351 WP_000624671.1 IS66 family insertion sequence element accessory protein TnpB -
C1P83_RS25310 152949..153305 - 357 WP_005054686.1 transposase -
C1P83_RS25315 153457..153621 - 165 Protein_188 IS630 family transposase -
C1P83_RS25325 154390..154863 + 474 Protein_190 conjugal transfer protein TraD -
C1P83_RS25330 154872..155270 - 399 WP_000911318.1 type II toxin-antitoxin system VapC family toxin Toxin
C1P83_RS25335 155270..155497 - 228 WP_000450530.1 toxin-antitoxin system antitoxin VapB Antitoxin

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospB / ospC4 / ospC4 / ospC4 / ospC4 / ospD2 / ospD2 / ospF / ospD1 / ipgB2 / ospD3/senA / ospC1 / senB / ospE2 / ospI / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / virA / icsA/virG / icsA/virG / papC / ipaH9.8 / ospC2 / nleE / icsP/sopA 1..181479 181479
- inside IScluster/Tn - ipaH9.8 128433..154140 25707


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14845.09 Da        Isoelectric Point: 8.5264

>T96148 WP_000911318.1 NZ_CP026812:c155270-154872 [Shigella flexneri]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRTEDWS

Download         Length: 399 bp

>T96148 NZ_CP026812:c155270-154872 [Shigella flexneri]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCTGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAACTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8535.59 Da        Isoelectric Point: 4.7513

>AT96148 WP_000450530.1 NZ_CP026812:c155497-155270 [Shigella flexneri]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHSVSADFMDNREQPGMQERESF

Download         Length: 228 bp

>AT96148 NZ_CP026812:c155497-155270 [Shigella flexneri]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAAGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6SD6

Antitoxin

Source ID Structure
AlphaFold DB Q31SG2

References