Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
| Location | 148033..148558 | Replicon | plasmid unnamed |
| Accession | NZ_CP026812 | ||
| Organism | Shigella flexneri strain 64-5500 | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | Q326Z8 |
| Locus tag | C1P83_RS25275 | Protein ID | WP_001159860.1 |
| Coordinates | 148033..148338 (-) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | Q7BEK0 |
| Locus tag | C1P83_RS25280 | Protein ID | WP_000813626.1 |
| Coordinates | 148340..148558 (-) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C1P83_RS25245 | 143068..143910 | - | 843 | WP_000931198.1 | polysaccharide deacetylase family protein | - |
| C1P83_RS25915 | 144274..144426 | - | 153 | WP_171763767.1 | hypothetical protein | - |
| C1P83_RS26515 | 144392..144544 | + | 153 | Protein_175 | hypothetical protein | - |
| C1P83_RS25250 | 144596..145293 | + | 698 | WP_226342160.1 | IS1 family transposase | - |
| C1P83_RS26520 | 145357..145526 | - | 170 | Protein_177 | type II toxin-antitoxin system toxin YacB | - |
| C1P83_RS25260 | 145526..145795 | - | 270 | WP_000079956.1 | type II toxin-antitoxin system antitoxin YacA | - |
| C1P83_RS25265 | 146003..147640 | - | 1638 | WP_032490759.1 | T3SS effector E3 ubiquitin-protein ligase IpaH9.8 | - |
| C1P83_RS25275 | 148033..148338 | - | 306 | WP_001159860.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
| C1P83_RS25280 | 148340..148558 | - | 219 | WP_000813626.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
| C1P83_RS26525 | 149151..149234 | + | 84 | Protein_182 | hypothetical protein | - |
| C1P83_RS25290 | 149243..149959 | - | 717 | Protein_183 | IS3-like element IS911 family transposase | - |
| C1P83_RS25295 | 150014..150945 | + | 932 | Protein_184 | IS3 family transposase | - |
| C1P83_RS25300 | 150979..152571 | - | 1593 | WP_001072385.1 | IS66 family transposase | - |
| C1P83_RS25305 | 152602..152952 | - | 351 | WP_000624671.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| C1P83_RS25310 | 152949..153305 | - | 357 | WP_005054686.1 | transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | ospB / ospC4 / ospC4 / ospC4 / ospC4 / ospD2 / ospD2 / ospF / ospD1 / ipgB2 / ospD3/senA / ospC1 / senB / ospE2 / ospI / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / virA / icsA/virG / icsA/virG / papC / ipaH9.8 / ospC2 / nleE / icsP/sopA | 1..181479 | 181479 | |
| - | inside | IScluster/Tn | - | ipaH9.8 | 128433..154140 | 25707 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11736.59 Da Isoelectric Point: 6.4674
>T96147 WP_001159860.1 NZ_CP026812:c148338-148033 [Shigella flexneri]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI
Download Length: 306 bp
>T96147 NZ_CP026812:c148338-148033 [Shigella flexneri]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGATCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGATCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8333.36 Da Isoelectric Point: 6.2838
>AT96147 WP_000813626.1 NZ_CP026812:c148558-148340 [Shigella flexneri]
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW
Download Length: 219 bp
>AT96147 NZ_CP026812:c148558-148340 [Shigella flexneri]
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4P7TTN3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q7BEK0 |