Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 37152..37909 Replicon plasmid unnamed
Accession NZ_CP026812
Organism Shigella flexneri strain 64-5500

Toxin (Protein)


Gene name tacT Uniprot ID Q31SN7
Locus tag C1P83_RS24555 Protein ID WP_000501969.1
Coordinates 37152..37637 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q31SN8
Locus tag C1P83_RS24560 Protein ID WP_011114751.1
Coordinates 37625..37909 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P83_RS25640 32653..32889 + 237 WP_158250172.1 transposase -
C1P83_RS24525 33024..33380 + 357 WP_004983733.1 transposase -
C1P83_RS24530 33377..33727 + 351 WP_000624671.1 IS66 family insertion sequence element accessory protein TnpB -
C1P83_RS24535 33758..35350 + 1593 WP_001072392.1 IS66 family transposase -
C1P83_RS24540 35386..35652 + 267 WP_241547467.1 transposase -
C1P83_RS25890 35703..35857 - 155 Protein_49 transposase domain-containing protein -
C1P83_RS24550 35940..37037 - 1098 Protein_50 group II intron reverse transcriptase/maturase -
C1P83_RS26490 36989..37144 + 156 Protein_51 hypothetical protein -
C1P83_RS24555 37152..37637 - 486 WP_000501969.1 GNAT family N-acetyltransferase Toxin
C1P83_RS24560 37625..37909 - 285 WP_011114751.1 DUF1778 domain-containing protein Antitoxin
C1P83_RS24575 38582..39750 + 1169 WP_134801051.1 IS3 family transposase -
C1P83_RS24580 39808..40934 - 1127 Protein_55 IS110-like element ISSso6 family transposase -
C1P83_RS24585 41433..42221 + 789 WP_005116773.1 AraC family invasion system transcriptional regulator VirF -
C1P83_RS24590 42214..42585 - 372 Protein_57 ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospB / ospC4 / ospC4 / ospC4 / ospC4 / ospD2 / ospD2 / ospF / ospD1 / ipgB2 / ospD3/senA / ospC1 / senB / ospE2 / ospI / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / virA / icsA/virG / icsA/virG / papC / ipaH9.8 / ospC2 / nleE / icsP/sopA 1..181479 181479
- inside IScluster/Tn - ospB / ospC4 / ospC4 / ospC4 / ospC4 / ospD2 / ospD2 / ospF / ospD1 / ipgB2 / ospD3/senA / ospC1 173..49228 49055


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(30-151)

Antitoxin

Vibrio phage ICP1, Orf50

(12-88)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17712.54 Da        Isoelectric Point: 10.1083

>T96146 WP_000501969.1 NZ_CP026812:c37637-37152 [Shigella flexneri]
MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Download         Length: 486 bp

>T96146 NZ_CP026812:c37637-37152 [Shigella flexneri]
ATGGGATGTGTAACTGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCGGTGAAGCTGTACTTGA
TGACTGGTTAAAGCAGAAGGGGCTAAAAAATCAGGCAATCGGAGCCACCCGGACTTTTGTTGTATGCAGAAAAGGTACGC
AACAAATAGTCGGTTTTTACTCACTGGCCACTGGTAGTGTCAATCATACAGAAGCTACAGGTAACCTTCGGCGCAATATG
CCAGACCCCATTCCTGTAATTATACTTGCACGCCTGGCTGTAGATGTGTCATTTCGAGGAAAAGGGCTTGGCGCGGATTT
ACTTCATGATGCAGTTCGTCGTTGTTACAGGGTTGCCGAAAATATCGGTGTGCGTGCAATTATGGTTCATGCGCTTACGG
AAAACGCAAAACAGTTTTATATTCATCATGGTTTTAAGCCATCAAAAACTCAGGTGCAAACATTGTTTCTTAAACTCCCT
CAATAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10871.52 Da        Isoelectric Point: 4.6812

>AT96146 WP_011114751.1 NZ_CP026812:c37909-37625 [Shigella flexneri]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Download         Length: 285 bp

>AT96146 NZ_CP026812:c37909-37625 [Shigella flexneri]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAGCTCAATCTCAGAGCAAGAGAATCACAGCGGATACTCATTGATGC
TGCTGCAGAAATTCTTCATAAGTCGCGTACAGATTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATGTTATTCTCG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATTGAAATGCTTGATGCTCCGGTTGCAGATGATCCT
GCCATCGAAAAATTGCTGGCAAGGAAACCTCAATGGGATGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q31SN7

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CUJ1

References