Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3886279..3886933 Replicon chromosome
Accession NZ_CP026811
Organism Shigella flexneri strain 64-5500

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag C1P83_RS20380 Protein ID WP_000244777.1
Coordinates 3886279..3886686 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C1P83_RS20385 Protein ID WP_000354046.1
Coordinates 3886667..3886933 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P83_RS20360 (3882236) 3882236..3883969 - 1734 WP_000813210.1 single-stranded-DNA-specific exonuclease RecJ -
C1P83_RS20365 (3883975) 3883975..3884685 - 711 WP_000715205.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C1P83_RS20370 (3884710) 3884710..3885606 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C1P83_RS20375 (3885718) 3885718..3886239 + 522 WP_001055874.1 flavodoxin FldB -
C1P83_RS20380 (3886279) 3886279..3886686 - 408 WP_000244777.1 protein YgfX Toxin
C1P83_RS20385 (3886667) 3886667..3886933 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C1P83_RS20390 (3887176) 3887176..3888156 + 981 WP_000886094.1 tRNA-modifying protein YgfZ -
C1P83_RS20395 (3888352) 3888352..3889011 - 660 WP_000250272.1 hemolysin III family protein -
C1P83_RS20400 (3889175) 3889175..3889486 - 312 WP_001182983.1 N(4)-acetylcytidine aminohydrolase -
C1P83_RS20405 (3889531) 3889531..3890964 + 1434 WP_004986559.1 6-phospho-beta-glucosidase BglA -
C1P83_RS20410 (3891021) 3891021..3891764 - 744 Protein_3939 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T96143 WP_000244777.1 NZ_CP026811:c3886686-3886279 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T96143 NZ_CP026811:c3886686-3886279 [Shigella flexneri]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGATTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT96143 WP_000354046.1 NZ_CP026811:c3886933-3886667 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT96143 NZ_CP026811:c3886933-3886667 [Shigella flexneri]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References