Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1254054..1254629 Replicon chromosome
Accession NZ_CP026811
Organism Shigella flexneri strain 64-5500

Toxin (Protein)


Gene name pemK Uniprot ID I6EL31
Locus tag C1P83_RS06790 Protein ID WP_000373726.1
Coordinates 1254054..1254371 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID I6EL32
Locus tag C1P83_RS06795 Protein ID WP_000288814.1
Coordinates 1254372..1254629 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P83_RS06760 (1250331) 1250331..1251593 + 1263 WP_000055693.1 integrase arm-type DNA-binding domain-containing protein -
C1P83_RS06775 (1252305) 1252305..1253189 - 885 WP_000511771.1 integrase domain-containing protein -
C1P83_RS25710 (1253748) 1253748..1253894 + 147 WP_000285696.1 hypothetical protein -
C1P83_RS06790 (1254054) 1254054..1254371 - 318 WP_000373726.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
C1P83_RS06795 (1254372) 1254372..1254629 - 258 WP_000288814.1 antitoxin Antitoxin
C1P83_RS06805 (1254978) 1254978..1255184 - 207 WP_000979502.1 helix-turn-helix transcriptional regulator -
C1P83_RS06810 (1255181) 1255181..1255642 - 462 WP_000201268.1 hypothetical protein -
C1P83_RS06815 (1256013) 1256013..1257617 - 1605 WP_000628841.1 LA2681 family HEPN domain-containing protein -
C1P83_RS06820 (1257630) 1257630..1257995 - 366 WP_000253098.1 hypothetical protein -
C1P83_RS06825 (1258023) 1258023..1258232 - 210 WP_001044499.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - flhA / cheZ / cheY / cheB / cheR / flhC / flhD 1241550..1280530 38980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-105)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11293.94 Da        Isoelectric Point: 10.2965

>T96141 WP_000373726.1 NZ_CP026811:c1254371-1254054 [Shigella flexneri]
MDRGEIWLVSLDPTTGHEQSGKRPVLIVSKASFNTFTRLPVVVPVTSGGNFAREAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARSGKRLERIPDAVVNEVLARL

Download         Length: 318 bp

>T96141 NZ_CP026811:c1254371-1254054 [Shigella flexneri]
ATGGACAGAGGAGAAATCTGGCTCGTTTCACTGGATCCCACCACTGGCCATGAACAAAGTGGAAAACGTCCGGTACTCAT
CGTATCGAAGGCATCGTTTAACACGTTCACCCGGTTGCCTGTTGTTGTCCCTGTCACCAGCGGCGGAAACTTTGCCCGCG
AAGCCGGTTTTACCGTTTCACTGGACGGGGCGGGAACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGGAGCTCGGAGCGGTAAGCGGCTGGAACGTATACCCGATGCTGTCGTGAATGAGGTGCTGGCTCGACTGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9280.76 Da        Isoelectric Point: 4.5821

>AT96141 WP_000288814.1 NZ_CP026811:c1254629-1254372 [Shigella flexneri]
MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT96141 NZ_CP026811:c1254629-1254372 [Shigella flexneri]
ATGTATACCACTCGCCTGAAAAAGGTTGGCGGATCCGTCATGCTGGCGGTTCCCCCCGCAGTGCTGAAAACGCTAGGGCT
GTCGACGGACAGCGAAGTGGGCATGACCATTGATAATGGCTGCCTGATTATTGAACCTCAAAAACGTCCCCGCTATTCGC
TCGAAGAACTGTTGGCGCAGTGCGATCCGCACGCTGAAATAAGCGAAGAGGGTCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATCCTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T2UX62

Antitoxin

Source ID Structure
AlphaFold DB A0A085HLS8

References