Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
| Location | 31124..31649 | Replicon | plasmid unnamed2 |
| Accession | NZ_CP026809 | ||
| Organism | Shigella dysenteriae strain 204/96 | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | Q326Z8 |
| Locus tag | C1P75_RS26035 | Protein ID | WP_001159860.1 |
| Coordinates | 31344..31649 (+) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | Q7BEK0 |
| Locus tag | C1P75_RS26030 | Protein ID | WP_000813626.1 |
| Coordinates | 31124..31342 (+) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C1P75_RS25985 | 26162..26269 | - | 108 | Protein_30 | ATP-binding protein | - |
| C1P75_RS25995 | 26488..27162 | + | 675 | WP_046891833.1 | IS66-like element accessory protein TnpA | - |
| C1P75_RS26000 | 27159..27323 | + | 165 | Protein_32 | IS66 family insertion sequence element accessory protein TnpB | - |
| C1P75_RS26005 | 27472..27633 | + | 162 | Protein_33 | IS110 family transposase | - |
| C1P75_RS26010 | 27706..28874 | + | 1169 | WP_094112608.1 | IS3 family transposase | - |
| C1P75_RS26015 | 28923..29195 | - | 273 | WP_000019158.1 | hypothetical protein | - |
| C1P75_RS26030 | 31124..31342 | + | 219 | WP_000813626.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
| C1P75_RS26035 | 31344..31649 | + | 306 | WP_001159860.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
| C1P75_RS26040 | 31673..31780 | + | 108 | WP_023592908.1 | transposase domain-containing protein | - |
| C1P75_RS26045 | 32042..33679 | + | 1638 | WP_000936802.1 | T3SS effector E3 ubiquitin-protein ligase IpaH9.8 | - |
| C1P75_RS26050 | 33887..34156 | + | 270 | WP_000079956.1 | type II toxin-antitoxin system antitoxin YacA | - |
| C1P75_RS28125 | 34156..34325 | + | 170 | Protein_42 | type II toxin-antitoxin system toxin YacB | - |
| C1P75_RS26060 | 34408..34890 | + | 483 | Protein_43 | type III secretion system effector protein kinase OspG | - |
| C1P75_RS26065 | 34908..35604 | - | 697 | Protein_44 | IS1 family transposase | - |
| C1P75_RS28130 | 35656..35808 | - | 153 | Protein_45 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | virA / icsA/virG / ipaH9.8 / ospG / ospC2 / nleE / icsP/sopA / ospB / ospD2 / ospF / ospD1 / ipgB2 / ospI / ospE2 / ipaH1.4 / senB / ospC1 / ospD3/senA / ipaH7.8 / ipaH4.5 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / spa40 | 1..226481 | 226481 | |
| - | inside | IScluster/Tn | - | ipaH9.8 / ospG | 5799..41532 | 35733 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11736.59 Da Isoelectric Point: 6.4674
>T96128 WP_001159860.1 NZ_CP026809:31344-31649 [Shigella dysenteriae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI
Download Length: 306 bp
>T96128 NZ_CP026809:31344-31649 [Shigella dysenteriae]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGATCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGATCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8333.36 Da Isoelectric Point: 6.2838
>AT96128 WP_000813626.1 NZ_CP026809:31124-31342 [Shigella dysenteriae]
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW
Download Length: 219 bp
>AT96128 NZ_CP026809:31124-31342 [Shigella dysenteriae]
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4P7TTN3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q7BEK0 |