Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 20429..20988 Replicon plasmid unnamed2
Accession NZ_CP026809
Organism Shigella dysenteriae strain 204/96

Toxin (Protein)


Gene name relE Uniprot ID D2AJT2
Locus tag C1P75_RS25930 Protein ID WP_000421262.1
Coordinates 20429..20704 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q327A8
Locus tag C1P75_RS25935 Protein ID WP_001178088.1
Coordinates 20704..20988 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P75_RS25900 15431..16644 + 1214 WP_167544920.1 IS3 family transposase -
C1P75_RS25910 16708..17838 + 1131 WP_050546159.1 IS91 family transposase -
C1P75_RS25915 18033..19124 - 1092 Protein_17 IS66 family transposase -
C1P75_RS25920 19132..19699 + 568 Protein_18 plasmid segregation protein ParM -
C1P75_RS28110 19702..19884 + 183 WP_241547534.1 plasmid partitioning/stability family protein -
C1P75_RS28115 20160..20297 + 138 WP_000766699.1 hypothetical protein -
C1P75_RS25930 20429..20704 - 276 WP_000421262.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C1P75_RS25935 20704..20988 - 285 WP_001178088.1 ribbon-helix-helix domain-containing protein Antitoxin
C1P75_RS25945 21440..21691 + 252 WP_000433928.1 transporter -
C1P75_RS25950 21741..21950 + 210 Protein_24 peptidoglycan-binding protein -
C1P75_RS28120 22032..22133 - 102 Protein_25 ATP-binding protein -
C1P75_RS25955 22285..22641 + 357 WP_005041107.1 transposase -
C1P75_RS25960 22638..22988 + 351 WP_000624671.1 IS66 family insertion sequence element accessory protein TnpB -
C1P75_RS25965 23019..24611 + 1593 WP_001072392.1 IS66 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - virA / icsA/virG / ipaH9.8 / ospG / ospC2 / nleE / icsP/sopA / ospB / ospD2 / ospF / ospD1 / ipgB2 / ospI / ospE2 / ipaH1.4 / senB / ospC1 / ospD3/senA / ipaH7.8 / ipaH4.5 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / spa40 1..226481 226481
- inside IScluster/Tn - ipaH9.8 / ospG 5799..41532 35733


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10766.48 Da        Isoelectric Point: 9.9324

>T96127 WP_000421262.1 NZ_CP026809:c20704-20429 [Shigella dysenteriae]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T96127 NZ_CP026809:c20704-20429 [Shigella dysenteriae]
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACAGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAGAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10497.93 Da        Isoelectric Point: 6.2150

>AT96127 WP_001178088.1 NZ_CP026809:c20988-20704 [Shigella dysenteriae]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDHPLPVPR

Download         Length: 285 bp

>AT96127 NZ_CP026809:c20988-20704 [Shigella dysenteriae]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACCATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PVM3

Antitoxin

Source ID Structure
AlphaFold DB Q327A8

References