Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 50360..50885 Replicon plasmid unnamed
Accession NZ_CP026806
Organism Shigella dysenteriae strain 2017C-4522

Toxin (Protein)


Gene name ccdB Uniprot ID Q326Z8
Locus tag C1P73_RS24640 Protein ID WP_001159860.1
Coordinates 50580..50885 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q7BEK0
Locus tag C1P73_RS24635 Protein ID WP_000813626.1
Coordinates 50360..50578 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P73_RS24605 45657..46787 - 1131 WP_050546159.1 IS91 family transposase -
C1P73_RS24620 48159..48431 - 273 WP_000019158.1 hypothetical protein -
C1P73_RS24630 48888..50116 - 1229 Protein_68 IS3-like element IS2 family transposase -
C1P73_RS24635 50360..50578 + 219 WP_000813626.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C1P73_RS24640 50580..50885 + 306 WP_001159860.1 type II toxin-antitoxin system toxin CcdB Toxin
C1P73_RS24645 50921..51016 + 96 Protein_71 transposase domain-containing protein -
C1P73_RS24650 51278..52915 + 1638 WP_129593020.1 T3SS effector E3 ubiquitin-protein ligase IpaH9.8 -
C1P73_RS24655 53123..53392 + 270 WP_000079956.1 type II toxin-antitoxin system antitoxin YacA -
C1P73_RS25885 53392..53561 + 170 Protein_74 type II toxin-antitoxin system toxin YacB -
C1P73_RS24665 53644..54126 + 483 Protein_75 type III secretion system effector protein kinase OspG -
C1P73_RS25890 54893..55045 - 153 Protein_77 hypothetical protein -
C1P73_RS25240 55011..55163 + 153 WP_171763767.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospC2 / nleE / icsP/sopA / icsP/sopA / ospB / ipaH9.8 / ospG 1..69966 69966
- inside IScluster/Tn - ospC2 / nleE / icsP/sopA / icsP/sopA / ospB / ipaH9.8 / ospG 5848..54437 48589


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11736.59 Da        Isoelectric Point: 6.4674

>T96102 WP_001159860.1 NZ_CP026806:50580-50885 [Shigella dysenteriae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T96102 NZ_CP026806:50580-50885 [Shigella dysenteriae]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGATCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8333.36 Da        Isoelectric Point: 6.2838

>AT96102 WP_000813626.1 NZ_CP026806:50360-50578 [Shigella dysenteriae]
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT96102 NZ_CP026806:50360-50578 [Shigella dysenteriae]
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTN3

Antitoxin

Source ID Structure
AlphaFold DB Q7BEK0

References