Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 14529..15304 Replicon plasmid unnamed
Accession NZ_CP026806
Organism Shigella dysenteriae strain 2017C-4522

Toxin (Protein)


Gene name tacT Uniprot ID Q326V1
Locus tag C1P73_RS24355 Protein ID WP_000405248.1
Coordinates 14822..15304 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q7BEG8
Locus tag C1P73_RS24350 Protein ID WP_010921625.1
Coordinates 14529..14831 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P73_RS24315 9625..10468 - 844 Protein_17 IS5-like element ISSfl7 family transposase -
C1P73_RS24320 10893..12347 + 1455 WP_000701106.1 type 3 secretion system effector OspC2 -
C1P73_RS24790 12496..12624 - 129 Protein_19 IS5/IS1182 family transposase -
C1P73_RS24335 12796..14004 - 1209 WP_046891828.1 IS91-like element ISSbo1 family transposase -
C1P73_RS24340 13985..14257 - 273 WP_000019158.1 hypothetical protein -
C1P73_RS24350 14529..14831 + 303 WP_010921625.1 DUF1778 domain-containing protein Antitoxin
C1P73_RS24355 14822..15304 + 483 WP_000405248.1 GNAT family N-acetyltransferase Toxin
C1P73_RS24360 15372..15895 + 524 Protein_24 transposase -
C1P73_RS24365 15938..16276 - 339 Protein_25 integrase core domain-containing protein -
C1P73_RS24370 16350..17517 + 1168 Protein_26 IS3 family transposase -
C1P73_RS24375 17541..18233 - 693 WP_000625268.1 type III secretion system effector cysteine methyltransferase OspZ -
C1P73_RS24380 18289..18441 + 153 Protein_28 IS3 family transposase -
C1P73_RS24905 18723..18860 - 138 WP_005026553.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospC2 / nleE / icsP/sopA / icsP/sopA / ospB / ipaH9.8 / ospG 1..69966 69966
- inside IScluster/Tn - ospC2 / nleE / icsP/sopA / icsP/sopA / ospB / ipaH9.8 / ospG 5848..54437 48589


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(29-149)

Antitoxin

Vibrio phage ICP1, Orf50

(19-94)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17736.50 Da        Isoelectric Point: 7.0382

>T96100 WP_000405248.1 NZ_CP026806:14822-15304 [Shigella dysenteriae]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Download         Length: 483 bp

>T96100 NZ_CP026806:14822-15304 [Shigella dysenteriae]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTGTTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11689.28 Da        Isoelectric Point: 4.9167

>AT96100 WP_010921625.1 NZ_CP026806:14529-14831 [Shigella dysenteriae]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Download         Length: 303 bp

>AT96100 NZ_CP026806:14529-14831 [Shigella dysenteriae]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090NWA7

Antitoxin

Source ID Structure
AlphaFold DB Q7BEG8

References