Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2252234..2252888 Replicon chromosome
Accession NZ_CP026805
Organism Shigella dysenteriae strain 2017C-4522

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag C1P73_RS12215 Protein ID WP_000244777.1
Coordinates 2252481..2252888 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C1P73_RS12210 Protein ID WP_000354046.1
Coordinates 2252234..2252500 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P73_RS12185 (2247522) 2247522..2248265 + 744 WP_000951947.1 SDR family oxidoreductase -
C1P73_RS12190 (2248322) 2248322..2249755 - 1434 WP_141095349.1 6-phospho-beta-glucosidase BglA -
C1P73_RS12195 (2249800) 2249800..2250111 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
C1P73_RS12200 (2250275) 2250275..2250934 + 660 WP_000250274.1 hemolysin III family protein -
C1P73_RS12205 (2251011) 2251011..2251991 - 981 WP_000886098.1 tRNA-modifying protein YgfZ -
C1P73_RS12210 (2252234) 2252234..2252500 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C1P73_RS12215 (2252481) 2252481..2252888 + 408 WP_000244777.1 protein YgfX Toxin
C1P73_RS12220 (2252928) 2252928..2253449 - 522 WP_001055874.1 flavodoxin FldB -
C1P73_RS12225 (2253561) 2253561..2254457 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C1P73_RS12230 (2254482) 2254482..2255192 + 711 WP_000715205.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C1P73_RS12235 (2255198) 2255198..2256930 + 1733 Protein_2365 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T96092 WP_000244777.1 NZ_CP026805:2252481-2252888 [Shigella dysenteriae]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T96092 NZ_CP026805:2252481-2252888 [Shigella dysenteriae]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGATTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT96092 WP_000354046.1 NZ_CP026805:2252234-2252500 [Shigella dysenteriae]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT96092 NZ_CP026805:2252234-2252500 [Shigella dysenteriae]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References