Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1834699..1835282 Replicon chromosome
Accession NZ_CP026805
Organism Shigella dysenteriae strain 2017C-4522

Toxin (Protein)


Gene name mazF Uniprot ID Q31XK8
Locus tag C1P73_RS10075 Protein ID WP_000254733.1
Coordinates 1834699..1835034 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag C1P73_RS10080 Protein ID WP_000581937.1
Coordinates 1835034..1835282 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P73_RS10055 (1830200) 1830200..1830526 + 327 Protein_1940 YgcG family protein -
C1P73_RS10060 (1830586) 1830586..1831884 - 1299 WP_000036723.1 phosphopyruvate hydratase -
C1P73_RS10065 (1831972) 1831972..1833609 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
C1P73_RS10070 (1833837) 1833837..1834628 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
C1P73_RS10075 (1834699) 1834699..1835034 - 336 WP_000254733.1 endoribonuclease MazF Toxin
C1P73_RS10080 (1835034) 1835034..1835282 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
C1P73_RS10085 (1835360) 1835360..1837594 - 2235 WP_000226815.1 GTP pyrophosphokinase -
C1P73_RS10090 (1837642) 1837642..1838943 - 1302 WP_000046825.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12167.15 Da        Isoelectric Point: 8.7218

>T96090 WP_000254733.1 NZ_CP026805:c1835034-1834699 [Shigella dysenteriae]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKR
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T96090 NZ_CP026805:c1835034-1834699 [Shigella dysenteriae]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGA
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT96090 WP_000581937.1 NZ_CP026805:c1835282-1835034 [Shigella dysenteriae]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT96090 NZ_CP026805:c1835282-1835034 [Shigella dysenteriae]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q31XK8

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References