Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 131151..131926 Replicon plasmid unnamed1
Accession NZ_CP026804
Organism Shigella flexneri strain 89-141

Toxin (Protein)


Gene name tacT Uniprot ID Q326V1
Locus tag C1P88_RS23975 Protein ID WP_000405248.1
Coordinates 131151..131633 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q7BEG8
Locus tag C1P88_RS23980 Protein ID WP_010921625.1
Coordinates 131624..131926 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P88_RS25720 126655..126732 + 78 Protein_148 hypothetical protein -
C1P88_RS23935 126805..127086 - 282 Protein_149 transposase domain-containing protein -
C1P88_RS23940 127141..128350 + 1210 Protein_150 IS3 family transposase -
C1P88_RS23950 128394..128525 + 132 Protein_151 transposase -
C1P88_RS23960 129858..130106 - 249 Protein_153 IS3 family transposase -
C1P88_RS23965 130180..130517 + 338 Protein_154 integrase core domain-containing protein -
C1P88_RS23970 130560..131083 - 524 Protein_155 transposase -
C1P88_RS23975 131151..131633 - 483 WP_000405248.1 GNAT family N-acetyltransferase Toxin
C1P88_RS23980 131624..131926 - 303 WP_010921625.1 DUF1778 domain-containing protein Antitoxin
C1P88_RS23990 132198..132470 + 273 WP_000019164.1 hypothetical protein -
C1P88_RS23995 132451..133659 + 1209 WP_134799754.1 IS91 family transposase -
C1P88_RS24005 133872..134680 + 809 WP_232627014.1 IS5-like element ISSfl7 family transposase -
C1P88_RS24010 134795..135826 + 1032 Protein_161 IS630 family transposase -
C1P88_RS24015 136020..136711 + 692 WP_134799770.1 IS1 family transposase -
C1P88_RS24020 136728..136907 - 180 Protein_163 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiA / mxiA / mxiA / mxiC / mxiC / mxiD / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ospC1 / ospD3/senA / ipaH4.5 / ipaH1.4 / ospE2 / ospE2 / ipaH2.5 / ipgB2 / ospD1 / ospF / ospD2 / ospC2 / ospC4 / ospC3 / ospB / icsP/sopA / nleE / ospC2 / ipaH7.8 / ospG / ipaH9.8 / ospI / papC / icsA/virG / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / mxiG / ipgF / ipgE / ipgD / ipgD / ipgD / ipgD / icsB / icsB / ipgA 1..245004 245004
- inside IScluster/Tn - ospC2 / ipaH7.8 121524..146905 25381


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(29-149)

Antitoxin

Vibrio phage ICP1, Orf50

(19-94)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17736.50 Da        Isoelectric Point: 7.0382

>T96073 WP_000405248.1 NZ_CP026804:c131633-131151 [Shigella flexneri]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Download         Length: 483 bp

>T96073 NZ_CP026804:c131633-131151 [Shigella flexneri]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTGTTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11689.28 Da        Isoelectric Point: 4.9167

>AT96073 WP_010921625.1 NZ_CP026804:c131926-131624 [Shigella flexneri]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Download         Length: 303 bp

>AT96073 NZ_CP026804:c131926-131624 [Shigella flexneri]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTTTGATGGCGGTAAAGCCTGAATGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090NWA7

Antitoxin

Source ID Structure
AlphaFold DB Q7BEG8

References