Detailed information of TA system TA096072

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 42478..43235
• Replicon: plasmid unnamed1
• Accession: NZ_CP026804
• Organism: Shigella flexneri strain 89-141

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: C1P88_RS23365
• Protein ID: WP_000501970.1
• Coordinates: 42750..43235 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: Q31SN8
• Locus tag: C1P88_RS23360
• Protein ID: WP_011114751.1
• Coordinates: 42478..42762 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P88_RS23325	38519..39193	+	675	WP_001088287.1	IS66-like element accessory protein TnpA	-
C1P88_RS23330	39190..39537	+	348	WP_000631715.1	IS66 family insertion sequence element accessory protein TnpB	-
C1P88_RS23335	39557..41158	+	1602	WP_005067462.1	IS66-like element ISSfl3 family transposase	-
C1P88_RS25110	41412..41576	-	165	WP_044138991.1	hypothetical protein	-
C1P88_RS23360	42478..42762	+	285	WP_011114751.1	DUF1778 domain-containing protein	Antitoxin
C1P88_RS23365	42750..43235	+	486	WP_000501970.1	GNAT family N-acetyltransferase	Toxin
C1P88_RS23370	43286..43983	+	698	WP_223368647.1	IS1 family transposase	-
C1P88_RS23375	44185..44505	-	321	Protein_47	IS4/IS5 family transposase	-
C1P88_RS23380	44568..45724	+	1157	WP_094184887.1	IS3-like element IS600 family transposase	-
C1P88_RS23385	45770..46090	+	321	Protein_49	ParB/RepB/Spo0J family partition protein	-
C1P88_RS23390	46318..46710	+	393	Protein_50	hypothetical protein	-
C1P88_RS23395	46729..47202	+	474	WP_005038443.1	AraC family transcriptional regulator	-
C1P88_RS23400	47291..48156	+	866	WP_086020993.1	IS5-like element ISSfl7 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiA / mxiA / mxiA / mxiC / mxiC / mxiD / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ospC1 / ospD3/senA / ipaH4.5 / ipaH1.4 / ospE2 / ospE2 / ipaH2.5 / ipgB2 / ospD1 / ospF / ospD2 / ospC2 / ospC4 / ospC3 / ospB / icsP/sopA / nleE / ospC2 / ipaH7.8 / ospG / ipaH9.8 / ospI / papC / icsA/virG / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / mxiG / ipgF / ipgE / ipgD / ipgD / ipgD / ipgD / icsB / icsB / ipgA	1..245004	245004
	-	inside	IScluster/Tn	-	ospC3 / ospC1 / ospD3/senA	30441..57274	26833

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (30-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (12-88)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17740.59 Da
• Isoelectric Point: 10.2962

>T96072 WP_000501970.1 NZ_CP026804:42750-43235 [Shigella flexneri]
MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
R

Nucleotide

• Length: 486 bp

>T96072 NZ_CP026804:42750-43235 [Shigella flexneri]
ATGGGATGTGTAACTGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCGGTGAAGCTGTACTTGA
TGACTGGTTAAAGCAGAAGGGGCTAAAAAATCAGGCAATCGGAGCCACCCGGACTTTTGTTGTATGCAGAAAAGGTACGC
AACAAATAGTCGGTTTTTACTCACTGGCCACTGGTAGTGTCAATCATACAGAAGCTACAGGTAACCTTCGGCGCAATATG
CCAGACCCCATTCCTGTAATTATACTTGCACGCCTGGCTGTAGATGTGTCATTTCGAGGAAAAGGGCTTGGCGCGGATTT
ACTTCATGATGCAGTTCGTCGTTGTTACAGGGTTGCCGAAAATATCGGTGTGCGTGCAATTATGGTTCATGCGCTTACGG
AAAACGCAAAACAGTTTTATATTCATCATGGTTTTAAGCCATCAAAAACTCAGGTGCAAACATTGTTTCTTAAACTCCCT
CGGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10871.52 Da
• Isoelectric Point: 4.6812

>AT96072 WP_011114751.1 NZ_CP026804:42478-42762 [Shigella flexneri]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Nucleotide

• Length: 285 bp

>AT96072 NZ_CP026804:42478-42762 [Shigella flexneri]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAGCTCAATCTCAGAGCAAGAGAATCACAGCGGATACTCATTGATGC
TGCTGCAGAAATTCTTCATAAGTCGCGTACAGATTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATGTTATTCTCG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATTGAAATGCTTGATGCTCCGGTTGCAGATGATCCT
GCCATCGAAAAATTGCTGGCAAGGAAACCTCAATGGGATGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A4V1CUJ1