Detailed information of TA system TA096069

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 2963407..2964244
• Replicon: chromosome
• Accession: NZ_CP026803
• Organism: Shigella flexneri strain 89-141

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: C1P88_RS15650
• Protein ID: WP_000227793.1
• Coordinates: 2963702..2964244 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A0A658YW11
• Locus tag: C1P88_RS15645
• Protein ID: WP_005072928.1
• Coordinates: 2963407..2963718 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P88_RS15620 (2960125)	2960125..2960739	+	615	WP_000179818.1	cytochrome o ubiquinol oxidase subunit III	-
C1P88_RS15625 (2960739)	2960739..2961068	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
C1P88_RS15630 (2961080)	2961080..2961745	+	666	Protein_2989	heme o synthase	-
C1P88_RS15640 (2962995)	2962995..2963222	+	228	Protein_2991	protoheme IX farnesyltransferase	-
C1P88_RS15645 (2963407)	2963407..2963718	+	312	WP_005072928.1	DUF1778 domain-containing protein	Antitoxin
C1P88_RS15650 (2963702)	2963702..2964244	+	543	WP_000227793.1	GNAT family N-acetyltransferase	Toxin
C1P88_RS15655 (2964300)	2964300..2965234	-	935	Protein_2994	sel1 repeat family protein	-
C1P88_RS15660 (2965245)	2965245..2965478	-	234	WP_128600802.1	hypothetical protein	-
C1P88_RS15665 (2965471)	2965471..2965764	-	294	WP_234426382.1	hypothetical protein	-
C1P88_RS15670 (2965806)	2965806..2966503	+	698	WP_094106546.1	IS1 family transposase	-
C1P88_RS15675 (2966509)	2966509..2967601	+	1093	Protein_2998	hypothetical protein	-
C1P88_RS15680 (2967654)	2967654..2968619	+	966	Protein_2999	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	2954953..2969138	14185

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (99-160)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19795.93 Da
• Isoelectric Point: 8.6178

>T96069 WP_000227793.1 NZ_CP026803:2963702-2964244 [Shigella flexneri]
MVDKHEEITLSIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLER
QRVSGVLQGSQPSEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T96069 NZ_CP026803:2963702-2964244 [Shigella flexneri]
ATGGTCGACAAGCATGAAGAGATTACTCTGTCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAGA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGGAGCGCG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAAAAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11410.11 Da
• Isoelectric Point: 10.3388

>AT96069 WP_005072928.1 NZ_CP026803:2963407-2963718 [Shigella flexneri]
MLGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT96069 NZ_CP026803:2963407-2963718 [Shigella flexneri]
ATGCTTGGAAAAACAGCCGCTCTATACGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A658YW11