Detailed information of TA system TA096067

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1797914..1798440
• Replicon: chromosome
• Accession: NZ_CP026803
• Organism: Shigella flexneri strain 89-141

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: C1P88_RS09365
• Protein ID: WP_000323025.1
• Coordinates: 1797914..1798201 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: C1P88_RS09370
• Protein ID: WP_000534858.1
• Coordinates: 1798201..1798440 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P88_RS09325 (1793363)	1793363..1794185	-	823	Protein_1783	antitermination protein	-
C1P88_RS09330 (1794200)	1794200..1794556	-	357	WP_000904111.1	RusA family crossover junction endodeoxyribonuclease	-
C1P88_RS09335 (1794569)	1794569..1795618	-	1050	WP_001265239.1	DUF968 domain-containing protein	-
C1P88_RS09340 (1795620)	1795620..1795898	-	279	WP_024258459.1	hypothetical protein	-
C1P88_RS25355 (1795965)	1795965..1796030	-	66	Protein_1787	hypothetical protein	-
C1P88_RS09350 (1796097)	1796097..1797265	+	1169	Protein_1788	IS3 family transposase	-
C1P88_RS09355 (1797200)	1797200..1797469	-	270	WP_000980985.1	hypothetical protein	-
C1P88_RS09360 (1797687)	1797687..1797842	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
C1P88_RS09365 (1797914)	1797914..1798201	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
C1P88_RS09370 (1798201)	1798201..1798440	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
C1P88_RS09375 (1798465)	1798465..1798749	+	285	WP_072039323.1	hypothetical protein	-
C1P88_RS09385 (1799996)	1799996..1800181	-	186	Protein_1795	YdeI family stress tolerance OB fold protein	-
C1P88_RS09395 (1801273)	1801273..1801791	+	519	WP_001024558.1	2-oxo-tetronate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1782897..1818508	35611
	-	inside	IScluster/Tn	-	-	1796097..1800703	4606

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T96067 WP_000323025.1 NZ_CP026803:c1798201-1797914 [Shigella flexneri]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T96067 NZ_CP026803:c1798201-1797914 [Shigella flexneri]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT96067 WP_000534858.1 NZ_CP026803:c1798440-1798201 [Shigella flexneri]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT96067 NZ_CP026803:c1798440-1798201 [Shigella flexneri]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGCGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8