Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 192966..193620 Replicon chromosome
Accession NZ_CP026803
Organism Shigella flexneri strain 89-141

Toxin (Protein)


Gene name cptA Uniprot ID Q0T101
Locus tag C1P88_RS01010 Protein ID WP_000244767.1
Coordinates 192966..193373 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID Q0T100
Locus tag C1P88_RS01015 Protein ID WP_000354044.1
Coordinates 193354..193620 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P88_RS00990 (188923) 188923..190656 - 1734 WP_000813187.1 single-stranded-DNA-specific exonuclease RecJ -
C1P88_RS00995 (190662) 190662..191372 - 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C1P88_RS01000 (191397) 191397..192293 - 897 WP_000806656.1 site-specific tyrosine recombinase XerD -
C1P88_RS01005 (192405) 192405..192926 + 522 WP_001055867.1 flavodoxin FldB -
C1P88_RS01010 (192966) 192966..193373 - 408 WP_000244767.1 protein YgfX Toxin
C1P88_RS01015 (193354) 193354..193620 - 267 WP_000354044.1 FAD assembly factor SdhE Antitoxin
C1P88_RS01020 (193863) 193863..194843 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
C1P88_RS01025 (195039) 195039..195698 - 660 WP_000250278.1 hemolysin III family protein -
C1P88_RS01030 (195862) 195862..196173 - 312 WP_001182938.1 N(4)-acetylcytidine aminohydrolase -
C1P88_RS01035 (196218) 196218..197651 + 1434 WP_005076748.1 6-phospho-beta-glucosidase BglA -
C1P88_RS01040 (197708) 197708..198451 - 744 WP_000951960.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15978.91 Da        Isoelectric Point: 10.9373

>T96054 WP_000244767.1 NZ_CP026803:c193373-192966 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T96054 NZ_CP026803:c193373-192966 [Shigella flexneri]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTTGTGGTATTTGATTGCG
TTCGCAGCCAGCGGTGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10529.09 Da        Isoelectric Point: 5.1599

>AT96054 WP_000354044.1 NZ_CP026803:c193620-193354 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT96054 NZ_CP026803:c193620-193354 [Shigella flexneri]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATAATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TIU0

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TIU2

References