Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3270580..3271077 Replicon chromosome
Accession NZ_CP026802
Organism Shigella sonnei strain ATCC 29930

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag C1P89_RS16990 Protein ID WP_001173343.1
Coordinates 3270580..3270753 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag C1P89_RS16995 Protein ID WP_000398619.1
Coordinates 3270781..3271077 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P89_RS16965 (3265732) 3265732..3266888 + 1157 WP_094096605.1 IS3-like element IS600 family transposase -
C1P89_RS16975 (3266934) 3266934..3267191 + 258 Protein_3263 lysine decarboxylase LdcC -
C1P89_RS16980 (3267250) 3267250..3268707 + 1458 WP_000856831.1 dipeptide/tripeptide permease DtpC -
C1P89_RS16985 (3268944) 3268944..3270461 + 1518 WP_001295086.1 lysine--tRNA ligase -
C1P89_RS16990 (3270580) 3270580..3270753 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
C1P89_RS16995 (3270781) 3270781..3271077 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
C1P89_RS17000 (3271304) 3271304..3271576 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
C1P89_RS17005 (3271588) 3271588..3271818 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
C1P89_RS17010 (3271999) 3271999..3273630 + 1632 WP_001216495.1 sensor histidine kinase -
C1P89_RS17015 (3273627) 3273627..3274346 + 720 WP_000611285.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T96049 WP_001173343.1 NZ_CP026802:c3270753-3270580 [Shigella sonnei]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T96049 NZ_CP026802:c3270753-3270580 [Shigella sonnei]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT96049 WP_000398619.1 NZ_CP026802:c3271077-3270781 [Shigella sonnei]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT96049 NZ_CP026802:c3271077-3270781 [Shigella sonnei]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References