Detailed information of TA system TA096047

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 2566031..2566868
• Replicon: chromosome
• Accession: NZ_CP026802
• Organism: Shigella sonnei strain ATCC 29930

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: C1P89_RS13560
• Protein ID: WP_000227791.1
• Coordinates: 2566326..2566868 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: C1P89_RS13555
• Protein ID: WP_001297137.1
• Coordinates: 2566031..2566342 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P89_RS13530 (2561051)	2561051..2561998	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
C1P89_RS13535 (2562020)	2562020..2564011	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
C1P89_RS13540 (2564001)	2564001..2564615	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
C1P89_RS13545 (2564615)	2564615..2564944	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
C1P89_RS13550 (2564956)	2564956..2565846	+	891	WP_000971327.1	heme o synthase	-
C1P89_RS13555 (2566031)	2566031..2566342	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
C1P89_RS13560 (2566326)	2566326..2566868	+	543	WP_000227791.1	GNAT family N-acetyltransferase	Toxin
C1P89_RS13565 (2566924)	2566924..2567490	-	567	Protein_2592	SEL1-like repeat protein	-
C1P89_RS13570 (2567524)	2567524..2568221	+	698	WP_237158176.1	IS1-like element IS1A family transposase	-
C1P89_RS13575 (2568238)	2568238..2568636	-	399	Protein_2594	SEL1-like repeat protein	-
C1P89_RS13585 (2569821)	2569821..2571185	+	1365	WP_001000971.1	MFS transporter	-
C1P89_RS13590 (2571313)	2571313..2571804	-	492	WP_001138904.1	nucleotide binding protein YajQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19794.98 Da
• Isoelectric Point: 8.3395

>T96047 WP_000227791.1 NZ_CP026802:2566326-2566868 [Shigella sonnei]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNSVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T96047 NZ_CP026802:2566326-2566868 [Shigella sonnei]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACTCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT96047 WP_001297137.1 NZ_CP026802:2566031-2566342 [Shigella sonnei]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT96047 NZ_CP026802:2566031-2566342 [Shigella sonnei]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6B3