Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2528555..2529173 Replicon chromosome
Accession NZ_CP026802
Organism Shigella sonnei strain ATCC 29930

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C1P89_RS13370 Protein ID WP_001291435.1
Coordinates 2528955..2529173 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C1P89_RS13365 Protein ID WP_000344800.1
Coordinates 2528555..2528929 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P89_RS13355 (2523644) 2523644..2524837 + 1194 WP_011310152.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C1P89_RS13360 (2524860) 2524860..2528009 + 3150 WP_001132464.1 efflux RND transporter permease AcrB -
C1P89_RS13365 (2528555) 2528555..2528929 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C1P89_RS13370 (2528955) 2528955..2529173 + 219 WP_001291435.1 HHA domain-containing protein Toxin
C1P89_RS13375 (2529344) 2529344..2529895 + 552 WP_000102564.1 maltose O-acetyltransferase -
C1P89_RS13380 (2530011) 2530011..2530481 + 471 WP_000136192.1 YlaC family protein -
C1P89_RS13385 (2530645) 2530645..2532193 + 1549 Protein_2556 cyclic-guanylate-specific phosphodiesterase PdeB -
C1P89_RS13390 (2532235) 2532235..2532588 - 354 WP_000878140.1 DUF1428 domain-containing protein -
C1P89_RS13400 (2532967) 2532967..2533278 + 312 WP_000409911.1 MGMT family protein -
C1P89_RS13405 (2533309) 2533309..2533881 - 573 WP_000779812.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T96046 WP_001291435.1 NZ_CP026802:2528955-2529173 [Shigella sonnei]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T96046 NZ_CP026802:2528955-2529173 [Shigella sonnei]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT96046 WP_000344800.1 NZ_CP026802:2528555-2528929 [Shigella sonnei]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT96046 NZ_CP026802:2528555-2528929 [Shigella sonnei]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References