Detailed information of TA system TA096035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 126046..126821
• Replicon: plasmid unnamed
• Accession: NZ_CP026800
• Organism: Shigella flexneri strain NCTC 9728

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q7BEG9
• Locus tag: C1P82_RS23785
• Protein ID: WP_000405245.1
• Coordinates: 126046..126528 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: Q7BEG8
• Locus tag: C1P82_RS23790
• Protein ID: WP_010921625.1
• Coordinates: 126519..126821 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P82_RS23770	122565..124256	+	1692	WP_134807744.1	T3SS effector E3 ubiquitin-protein ligase IpaH2.5	-
C1P82_RS23775	124308..125412	+	1105	Protein_150	IS3 family transposase	-
C1P82_RS23780	125455..125978	-	524	Protein_151	transposase	-
C1P82_RS23785	126046..126528	-	483	WP_000405245.1	GNAT family N-acetyltransferase	Toxin
C1P82_RS23790	126519..126821	-	303	WP_010921625.1	DUF1778 domain-containing protein	Antitoxin
C1P82_RS23800	127093..127365	+	273	WP_000019158.1	hypothetical protein	-
C1P82_RS23805	127346..128554	+	1209	WP_134807736.1	IS91 family transposase	-
C1P82_RS23935	128726..128854	+	129	Protein_156	IS5/IS1182 family transposase	-
C1P82_RS23820	129003..130457	-	1455	WP_010921627.1	type 3 secretion system effector OspC2	-
C1P82_RS23825	130861..131504	+	644	Protein_158	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ipaH7.8 / ipaH4.5 / ospD3/senA / ospC1 / ospI / ipaH9.8 / ospG / ipaH1.4 / ospE1 / nleE / icsP/sopA / ospB / ospC4 / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ospC2 / espC	1..135368	135368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (29-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17750.52 Da
• Isoelectric Point: 7.0382

>T96035 WP_000405245.1 NZ_CP026800:c126528-126046 [Shigella flexneri]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Nucleotide

• Length: 483 bp

>T96035 NZ_CP026800:c126528-126046 [Shigella flexneri]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTATTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11689.28 Da
• Isoelectric Point: 4.9167

>AT96035 WP_010921625.1 NZ_CP026800:c126821-126519 [Shigella flexneri]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Nucleotide

• Length: 303 bp

>AT96035 NZ_CP026800:c126821-126519 [Shigella flexneri]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Structures

Toxin

• AlphaFold DB: Q7BEG9

Antitoxin

• AlphaFold DB: Q7BEG8