Detailed information of TA system TA096032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 28203..28960
• Replicon: plasmid unnamed
• Accession: NZ_CP026800
• Organism: Shigella flexneri strain NCTC 9728

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q99QI3
• Locus tag: C1P82_RS23110
• Protein ID: WP_010921647.1
• Coordinates: 28203..28688 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: Q31SN8
• Locus tag: C1P82_RS23115
• Protein ID: WP_011114751.1
• Coordinates: 28676..28960 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P82_RS23075	23293..23462	-	170	Protein_24	HNH endonuclease	-
C1P82_RS23085	24690..25163	-	474	WP_005038443.1	AraC family transcriptional regulator	-
C1P82_RS23090	25151..25579	-	429	WP_010921645.1	hypothetical protein	-
C1P82_RS23095	25807..26445	-	639	WP_000502855.1	ParB N-terminal domain-containing protein	-
C1P82_RS23100	26430..26573	-	144	Protein_29	DUF3440 domain-containing protein	-
C1P82_RS23110	28203..28688	-	486	WP_010921647.1	GNAT family N-acetyltransferase	Toxin
C1P82_RS23115	28676..28960	-	285	WP_011114751.1	DUF1778 domain-containing protein	Antitoxin
C1P82_RS24250	29862..30026	+	165	WP_001346193.1	hypothetical protein	-
C1P82_RS23140	30280..31876	-	1597	Protein_34	IS66-like element ISSfl3 family transposase	-
C1P82_RS23145	31873..32223	-	351	WP_005048975.1	IS66 family insertion sequence element accessory protein TnpB	-
C1P82_RS23150	32220..32894	-	675	WP_004967157.1	IS66 family insertion sequence hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ipaH7.8 / ipaH4.5 / ospD3/senA / ospC1 / ospI / ipaH9.8 / ospG / ipaH1.4 / ospE1 / nleE / icsP/sopA / ospB / ospC4 / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ospC2 / espC	1..135368	135368
	-	inside	IScluster/Tn	-	ipaH7.8 / ipaH4.5 / ospD3/senA / ospC1 / ospI / ipaH9.8 / ospG	5038..47368	42330

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (12-88)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17784.60 Da
• Isoelectric Point: 9.8822

>T96032 WP_010921647.1 NZ_CP026800:c28688-28203 [Shigella flexneri]
MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKELKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T96032 NZ_CP026800:c28688-28203 [Shigella flexneri]
ATGGGATGTGTAACTGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCGGTGAAGCTGTACTTGA
TGACTGGTTAAAGCAGAAGGAGCTAAAAAATCAGGCAATCGGAGCCACCCGGACTTTTGTTGTATGCAGAAAAGGTACGC
AACAAATAGTCGGTTTTTACTCACTGGCCACTGGTAGTGTCAATCATACAGAAGCTACAGGTAACCTTCGGCGCAATATG
CCAGACCCCATTCCTGTAATTATACTTGCACGCCTGGCTGTAGATGTGTCATTTCGAGGAAAAGGGCTTGGCGCGGATTT
ACTTCATGATGCAGTTCGTCGTTGTTACAGGGTTGCCGAAAATATCGGTGTGCGTGCAATTATGGTTCATGCGCTTACGG
AAAACGCAAAACAGTTTTATATTCATCATGGTTTTAAGCCATCAAAAACTCAGGTGCAAACATTGTTTCTTAAACTCCCT
CAATAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10871.52 Da
• Isoelectric Point: 4.6812

>AT96032 WP_011114751.1 NZ_CP026800:c28960-28676 [Shigella flexneri]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Nucleotide

• Length: 285 bp

>AT96032 NZ_CP026800:c28960-28676 [Shigella flexneri]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAGCTCAATCTCAGAGCAAGAGAATCACAGCGGATACTCATTGATGC
TGCTGCAGAAATTCTTCATAAGTCGCGTACAGATTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATGTTATTCTCG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATTGAAATGCTTGATGCTCCGGTTGCAGATGATCCT
GCCATCGAAAAATTGCTGGCAAGGAAACCTCAATGGGATGTGTAA

Structures

Toxin

• AlphaFold DB: A0A4P7TUC3

Antitoxin

• AlphaFold DB: A0A4V1CUJ1