Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 28203..28960 Replicon plasmid unnamed
Accession NZ_CP026800
Organism Shigella flexneri strain NCTC 9728

Toxin (Protein)


Gene name tacT Uniprot ID Q99QI3
Locus tag C1P82_RS23110 Protein ID WP_010921647.1
Coordinates 28203..28688 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q31SN8
Locus tag C1P82_RS23115 Protein ID WP_011114751.1
Coordinates 28676..28960 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P82_RS23075 23293..23462 - 170 Protein_24 HNH endonuclease -
C1P82_RS23085 24690..25163 - 474 WP_005038443.1 AraC family transcriptional regulator -
C1P82_RS23090 25151..25579 - 429 WP_010921645.1 hypothetical protein -
C1P82_RS23095 25807..26445 - 639 WP_000502855.1 ParB N-terminal domain-containing protein -
C1P82_RS23100 26430..26573 - 144 Protein_29 DUF3440 domain-containing protein -
C1P82_RS23110 28203..28688 - 486 WP_010921647.1 GNAT family N-acetyltransferase Toxin
C1P82_RS23115 28676..28960 - 285 WP_011114751.1 DUF1778 domain-containing protein Antitoxin
C1P82_RS24250 29862..30026 + 165 WP_001346193.1 hypothetical protein -
C1P82_RS23140 30280..31876 - 1597 Protein_34 IS66-like element ISSfl3 family transposase -
C1P82_RS23145 31873..32223 - 351 WP_005048975.1 IS66 family insertion sequence element accessory protein TnpB -
C1P82_RS23150 32220..32894 - 675 WP_004967157.1 IS66 family insertion sequence hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ipaH7.8 / ipaH4.5 / ospD3/senA / ospC1 / ospI / ipaH9.8 / ospG / ipaH1.4 / ospE1 / nleE / icsP/sopA / ospB / ospC4 / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ospC2 / espC 1..135368 135368
- inside IScluster/Tn - ipaH7.8 / ipaH4.5 / ospD3/senA / ospC1 / ospI / ipaH9.8 / ospG 5038..47368 42330


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-151)

Antitoxin

Vibrio phage ICP1, Orf50

(12-88)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17784.60 Da        Isoelectric Point: 9.8822

>T96032 WP_010921647.1 NZ_CP026800:c28688-28203 [Shigella flexneri]
MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKELKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Download         Length: 486 bp

>T96032 NZ_CP026800:c28688-28203 [Shigella flexneri]
ATGGGATGTGTAACTGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCGGTGAAGCTGTACTTGA
TGACTGGTTAAAGCAGAAGGAGCTAAAAAATCAGGCAATCGGAGCCACCCGGACTTTTGTTGTATGCAGAAAAGGTACGC
AACAAATAGTCGGTTTTTACTCACTGGCCACTGGTAGTGTCAATCATACAGAAGCTACAGGTAACCTTCGGCGCAATATG
CCAGACCCCATTCCTGTAATTATACTTGCACGCCTGGCTGTAGATGTGTCATTTCGAGGAAAAGGGCTTGGCGCGGATTT
ACTTCATGATGCAGTTCGTCGTTGTTACAGGGTTGCCGAAAATATCGGTGTGCGTGCAATTATGGTTCATGCGCTTACGG
AAAACGCAAAACAGTTTTATATTCATCATGGTTTTAAGCCATCAAAAACTCAGGTGCAAACATTGTTTCTTAAACTCCCT
CAATAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10871.52 Da        Isoelectric Point: 4.6812

>AT96032 WP_011114751.1 NZ_CP026800:c28960-28676 [Shigella flexneri]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Download         Length: 285 bp

>AT96032 NZ_CP026800:c28960-28676 [Shigella flexneri]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAGCTCAATCTCAGAGCAAGAGAATCACAGCGGATACTCATTGATGC
TGCTGCAGAAATTCTTCATAAGTCGCGTACAGATTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATGTTATTCTCG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATTGAAATGCTTGATGCTCCGGTTGCAGATGATCCT
GCCATCGAAAAATTGCTGGCAAGGAAACCTCAATGGGATGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TUC3

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CUJ1

References