Detailed information of TA system TA096030

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3250259..3251103
• Replicon: chromosome
• Accession: NZ_CP026799
• Organism: Shigella flexneri strain NCTC 9728

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: A0A4P7TPN5
• Locus tag: C1P82_RS16865
• Protein ID: WP_005060796.1
• Coordinates: 3250705..3251103 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: -
• Locus tag: C1P82_RS16855
• Protein ID: WP_134807688.1
• Coordinates: 3250259..3250570 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P82_RS16830	3245729..3246226	+	498	Protein_3218	COX aromatic rich motif-containing protein	-
C1P82_RS16835	3246248..3248239	+	1992	Protein_3219	cytochrome o ubiquinol oxidase subunit I	-
C1P82_RS16840	3248229..3248843	+	615	Protein_3220	cytochrome o ubiquinol oxidase subunit III	-
C1P82_RS16845	3248843..3249172	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
C1P82_RS16850	3249184..3250074	+	891	Protein_3222	protoheme IX farnesyltransferase	-
C1P82_RS16855	3250259..3250570	+	312	WP_134807688.1	DUF1778 domain-containing protein	Antitoxin
C1P82_RS16865	3250705..3251103	+	399	WP_005060796.1	GNAT family N-acetyltransferase	Toxin
C1P82_RS16870	3251159..3252094	-	936	WP_005105029.1	sel1 repeat family protein	-
C1P82_RS16875	3252105..3252338	-	234	WP_128600802.1	hypothetical protein	-
C1P82_RS16880	3252331..3253605	-	1275	WP_134807689.1	sel1 repeat family protein	-
C1P82_RS16885	3253999..3255369	+	1371	WP_001000961.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (52-112)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14335.69 Da
• Isoelectric Point: 9.1674

>T96030 WP_005060796.1 NZ_CP026799:3250705-3251103 [Shigella flexneri]
VRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKK
IHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 399 bp

>T96030 NZ_CP026799:3250705-3251103 [Shigella flexneri]
GTGCGTAATAGCGACTGTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGC
ATATTCGCTGGAAAGACAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCA
TGTTGGGGGGAGCGCGGAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAAAA
ATTCACCCGGCATTACCAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTT
TGTTCAGCTTTCAGCGACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11424.10 Da
• Isoelectric Point: 10.3388

>AT96030 WP_134807688.1 NZ_CP026799:3250259-3250570 [Shigella flexneri]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIT
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT96030 NZ_CP026799:3250259-3250570 [Shigella flexneri]
ATGCCTGGAAAAACAGCCGCTCTATACGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCACT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A4P7TPN5