Detailed information of TA system TA096028

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2045517..2046043
• Replicon: chromosome
• Accession: NZ_CP026799
• Organism: Shigella flexneri strain NCTC 9728

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: C1P82_RS10445
• Protein ID: WP_000323025.1
• Coordinates: 2045756..2046043 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: C1P82_RS10440
• Protein ID: WP_000534858.1
• Coordinates: 2045517..2045756 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P82_RS10415	2041314..2042523	-	1210	Protein_1985	IS3 family transposase	-
C1P82_RS10420	2042597..2043271	-	675	WP_134807626.1	IS66-like element accessory protein TnpA	-
C1P82_RS10425	2043434..2043979	+	546	Protein_1987	hypothetical protein	-
C1P82_RS10430	2044044..2045190	+	1147	Protein_1988	IS3 family transposase	-
C1P82_RS10435	2045187..2045492	-	306	WP_071818640.1	hypothetical protein	-
C1P82_RS10440	2045517..2045756	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
C1P82_RS10445	2045756..2046043	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
C1P82_RS10450	2046115..2046270	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
C1P82_RS10455	2046488..2046739	+	252	WP_000980987.1	hypothetical protein	-
C1P82_RS10460	2046806..2047084	+	279	WP_024258459.1	hypothetical protein	-
C1P82_RS10465	2047086..2048136	+	1051	Protein_1995	DUF968 domain-containing protein	-
C1P82_RS10470	2048149..2048523	+	375	WP_000904117.1	RusA family crossover junction endodeoxyribonuclease	-
C1P82_RS24125	2048520..2049341	+	822	Protein_1997	antitermination protein	-
C1P82_RS10500	2050261..2050369	+	109	Protein_1998	DUF3927 family protein	-
C1P82_RS10505	2050650..2050985	-	336	WP_000871291.1	anti-adapter protein IraM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2040717..2062136	21419

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T96028 WP_000323025.1 NZ_CP026799:2045756-2046043 [Shigella flexneri]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T96028 NZ_CP026799:2045756-2046043 [Shigella flexneri]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT96028 WP_000534858.1 NZ_CP026799:2045517-2045756 [Shigella flexneri]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT96028 NZ_CP026799:2045517-2045756 [Shigella flexneri]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• AlphaFold DB: A0A4V1CTS8