Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 213433..214058 Replicon plasmid unnamed
Accession NZ_CP026796
Organism Shigella boydii strain ATCC BAA-1247

Toxin (Protein)


Gene name vapC Uniprot ID A0A2S8DLI0
Locus tag C5B80_RS25620 Protein ID WP_000911319.1
Coordinates 213660..214058 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q31SG2
Locus tag C5B80_RS25615 Protein ID WP_000450530.1
Coordinates 213433..213660 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5B80_RS25610 212936..213229 - 294 WP_171548087.1 relaxase domain-containing protein -
C5B80_RS25615 213433..213660 + 228 WP_000450530.1 toxin-antitoxin system antitoxin VapB Antitoxin
C5B80_RS25620 213660..214058 + 399 WP_000911319.1 tRNA(fMet)-specific endonuclease VapC Toxin
C5B80_RS25625 214067..216255 - 2189 Protein_253 type IV conjugative transfer system coupling protein TraD -
C5B80_RS25630 216503..216676 + 174 Protein_254 IS1 family transposase -
C5B80_RS25635 216727..217940 + 1214 WP_164997312.1 IS3 family transposase -
C5B80_RS25640 217984..218513 + 530 Protein_256 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospG / ipaH9.8 / papC / icsA/virG / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ipaH4.5 / ipaH7.8 / senB / ospC1 / ospD3/senA / ospD3/senA / ospE2 / ipaH1.4 / ospI / ipgB2 / ospD1 / ospF / ospD2 / ospD2 / ospC4 / ospB / icsP/sopA / icsP/sopA / nleE / ospC2 / ospG / ipaH9.8 / ipaH9.8 1..226559 226559


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14887.17 Da        Isoelectric Point: 8.5264

>T96012 WP_000911319.1 NZ_CP026796:213660-214058 [Shigella boydii]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
VQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRTEDWS

Download         Length: 399 bp

>T96012 NZ_CP026796:213660-214058 [Shigella boydii]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCTGCAGCCACACACACC
GTCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAACTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8535.59 Da        Isoelectric Point: 4.7513

>AT96012 WP_000450530.1 NZ_CP026796:213433-213660 [Shigella boydii]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHSVSADFMDNREQPGMQERESF

Download         Length: 228 bp

>AT96012 NZ_CP026796:213433-213660 [Shigella boydii]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S8DLI0

Antitoxin

Source ID Structure
AlphaFold DB Q31SG2

References