Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 148306..149063 Replicon plasmid unnamed
Accession NZ_CP026796
Organism Shigella boydii strain ATCC BAA-1247

Toxin (Protein)


Gene name tacT Uniprot ID Q31SN7
Locus tag C5B80_RS25150 Protein ID WP_000501969.1
Coordinates 148578..149063 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q31SN8
Locus tag C5B80_RS25145 Protein ID WP_011114751.1
Coordinates 148306..148590 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5B80_RS26185 143743..144381 + 639 WP_000607008.1 type III secretion system effector OspI -
C5B80_RS25110 144496..144765 + 270 Protein_167 transposase -
C5B80_RS25115 144853..146081 + 1229 WP_167549346.1 IS3-like element IS2 family transposase -
C5B80_RS25125 146329..146455 + 127 Protein_169 IS110 family transposase -
C5B80_RS25145 148306..148590 + 285 WP_011114751.1 DUF1778 domain-containing protein Antitoxin
C5B80_RS25150 148578..149063 + 486 WP_000501969.1 GNAT family N-acetyltransferase Toxin
C5B80_RS25155 149104..150696 - 1593 WP_001072392.1 IS66 family transposase -
C5B80_RS25160 150727..151077 - 351 Protein_174 IS66 family insertion sequence element accessory protein TnpB -
C5B80_RS25165 151074..151841 - 768 Protein_175 transposase -
C5B80_RS25170 152143..153356 + 1214 WP_171766231.1 IS3 family transposase -
C5B80_RS25175 153393..153572 + 180 Protein_177 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospG / ipaH9.8 / papC / icsA/virG / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ipaH4.5 / ipaH7.8 / senB / ospC1 / ospD3/senA / ospD3/senA / ospE2 / ipaH1.4 / ospI / ipgB2 / ospD1 / ospF / ospD2 / ospD2 / ospC4 / ospB / icsP/sopA / icsP/sopA / nleE / ospC2 / ospG / ipaH9.8 / ipaH9.8 1..226559 226559
- inside IScluster/Tn - ospC1 / ospD3/senA / ospD3/senA / ospE2 / ipaH1.4 / ospI 119684..153581 33897


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(30-151)

Antitoxin

Vibrio phage ICP1, Orf50

(12-88)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17712.54 Da        Isoelectric Point: 10.1083

>T96010 WP_000501969.1 NZ_CP026796:148578-149063 [Shigella boydii]
MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Download         Length: 486 bp

>T96010 NZ_CP026796:148578-149063 [Shigella boydii]
ATGGGATGTGTAACTGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCGGTGAAGCTGTACTTGA
TGACTGGTTAAAGCAGAAGGGGCTAAAAAATCAGGCAATCGGAGCCACCCGGACTTTTGTTGTATGCAGAAAAGGTACGC
AACAAATAGTCGGTTTTTACTCACTGGCCACTGGTAGTGTCAATCATACAGAAGCTACAGGTAACCTTCGGCGCAATATG
CCAGACCCCATTCCTGTAATTATACTTGCACGCCTGGCTGTAGATGTGTCATTTCGAGGAAAAGGGCTTGGCGCGGATTT
ACTTCATGATGCAGTTCGTCGTTGTTACAGGGTTGCCGAAAATATCGGTGTGCGTGCAATTATGGTTCATGCGCTTACGG
AAAACGCAAAACAGTTTTATATTCATCATGGTTTTAAGCCATCAAAAACTCAGGTGCAAACATTGTTTCTTAAACTCCCT
CAATAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10871.52 Da        Isoelectric Point: 4.6812

>AT96010 WP_011114751.1 NZ_CP026796:148306-148590 [Shigella boydii]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Download         Length: 285 bp

>AT96010 NZ_CP026796:148306-148590 [Shigella boydii]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAGCTCAATCTCAGAGCAAGAGAATCACAGCGGATACTCATTGATGC
TGCTGCAGAAATTCTTCATAAGTCGCGTACAGATTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATGTTATTCTCG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATTGAAATGCTTGATGCTCCGGTTGCAGATGATCCT
GCCATCGAAAAATTGCTGGCAAGGAAACCTCAATGGGATGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q31SN7

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CUJ1

References