Detailed information of TA system TA095994

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 216454..217229
• Replicon: plasmid unnamed
• Accession: NZ_CP026794
• Organism: Shigella flexneri strain 74-1170

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q7BEG9
• Locus tag: C1P81_RS25855
• Protein ID: WP_000405245.1
• Coordinates: 216454..216936 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: Q7BEG8
• Locus tag: C1P81_RS25860
• Protein ID: WP_010921625.1
• Coordinates: 216927..217229 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P81_RS25840	212869..214560	+	1692	Protein_258	E3 ubiquitin--protein ligase	-
C1P81_RS25845	214612..215820	+	1209	Protein_259	IS3 family transposase	-
C1P81_RS25850	215863..216386	-	524	Protein_260	IS3-like element ISSfl11 family transposase	-
C1P81_RS25855	216454..216936	-	483	WP_000405245.1	GNAT family N-acetyltransferase	Toxin
C1P81_RS25860	216927..217229	-	303	WP_010921625.1	DUF1778 domain-containing protein	Antitoxin
C1P81_RS25870	217501..217773	+	273	WP_000019158.1	hypothetical protein	-
C1P81_RS25875	217754..218962	+	1209	WP_000198554.1	IS91 family transposase	-
C1P81_RS26150	219134..219262	+	129	Protein_265	IS5/IS1182 family transposase	-
C1P81_RS25890	219411..220865	-	1455	WP_000701108.1	type 3 secretion system effector OspC2	-
C1P81_RS25895	221269..221888	+	620	Protein_267	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ipaH7.8 / ipaH7.8 / ipaH4.5 / ipaH4.5 / ipaH4.5 / ipaH1.4 / ospD3/senA / ospC1 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG / ipaH1.4 / ospE1 / nleE / icsP/sopA / ospB / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ipaH7.8 / ospC2 / ipaH7.8 / ipaH4.5 / ospD3/senA / ospD3/senA / ospC1 / ospC1	1..251323	251323
	-	inside	IScluster/Tn	-	ospE2 / ipaH2.5 / ipaH7.8 / ospC2 / ipaH7.8 / ipaH4.5 / ospD3/senA / ospD3/senA / ospC1 / ospC1	201488..251102	49614

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (29-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17750.52 Da
• Isoelectric Point: 7.0382

>T95994 WP_000405245.1 NZ_CP026794:c216936-216454 [Shigella flexneri]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Nucleotide

• Length: 483 bp

>T95994 NZ_CP026794:c216936-216454 [Shigella flexneri]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTATTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11689.28 Da
• Isoelectric Point: 4.9167

>AT95994 WP_010921625.1 NZ_CP026794:c217229-216927 [Shigella flexneri]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Nucleotide

• Length: 303 bp

>AT95994 NZ_CP026794:c217229-216927 [Shigella flexneri]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Structures

Toxin

• AlphaFold DB: Q7BEG9

Antitoxin

• AlphaFold DB: Q7BEG8