Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 123438..123997 | Replicon | plasmid unnamed |
| Accession | NZ_CP026794 | ||
| Organism | Shigella flexneri strain 74-1170 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | D2AJT2 |
| Locus tag | C1P81_RS25220 | Protein ID | WP_000421262.1 |
| Coordinates | 123722..123997 (+) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | A0A822PWA0 |
| Locus tag | C1P81_RS25215 | Protein ID | WP_011114774.1 |
| Coordinates | 123438..123722 (+) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C1P81_RS25180 | 119383..120057 | + | 675 | WP_134799904.1 | IS66-like element accessory protein TnpA | - |
| C1P81_RS25185 | 120054..120404 | + | 351 | WP_005048975.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| C1P81_RS25190 | 120401..122002 | + | 1602 | WP_011110603.1 | IS66-like element ISSfl3 family transposase | - |
| C1P81_RS25195 | 122191..122376 | + | 186 | Protein_141 | ATP-binding protein | - |
| C1P81_RS25200 | 122476..122685 | - | 210 | Protein_142 | peptidoglycan-binding protein | - |
| C1P81_RS25205 | 122735..122986 | - | 252 | WP_001381727.1 | transporter | - |
| C1P81_RS25215 | 123438..123722 | + | 285 | WP_011114774.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| C1P81_RS25220 | 123722..123997 | + | 276 | WP_000421262.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| C1P81_RS25235 | 124976..125929 | + | 954 | Protein_147 | IS66 family transposase | - |
| C1P81_RS25240 | 126465..126683 | + | 219 | WP_000813626.1 | type II toxin-antitoxin system antitoxin CcdA | - |
| C1P81_RS25245 | 126685..126990 | + | 306 | WP_001159860.1 | type II toxin-antitoxin system toxin CcdB | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | ipaH7.8 / ipaH7.8 / ipaH4.5 / ipaH4.5 / ipaH4.5 / ipaH1.4 / ospD3/senA / ospC1 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG / ipaH1.4 / ospE1 / nleE / icsP/sopA / ospB / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ipaH7.8 / ospC2 / ipaH7.8 / ipaH4.5 / ospD3/senA / ospD3/senA / ospC1 / ospC1 | 1..251323 | 251323 | |
| - | inside | IScluster/Tn | - | ospI / ipaH9.8 / ospG | 111690..133560 | 21870 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10766.48 Da Isoelectric Point: 9.9324
>T95991 WP_000421262.1 NZ_CP026794:123722-123997 [Shigella flexneri]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T95991 NZ_CP026794:123722-123997 [Shigella flexneri]
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAGAACAGGTAG
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAGAACAGGTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10529.97 Da Isoelectric Point: 6.2150
>AT95991 WP_011114774.1 NZ_CP026794:123438-123722 [Shigella flexneri]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSW
ADSLSTDHLLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSW
ADSLSTDHLLPVPR
Download Length: 285 bp
>AT95991 NZ_CP026794:123438-123722 [Shigella flexneri]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGTCCTGG
GCGGACAGCCTCAGTACTGACCATCTGTTACCGGTGCCACGCTGA
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGTCCTGG
GCGGACAGCCTCAGTACTGACCATCTGTTACCGGTGCCACGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A822PVM3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A822PWA0 |