Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 123438..123997 Replicon plasmid unnamed
Accession NZ_CP026794
Organism Shigella flexneri strain 74-1170

Toxin (Protein)


Gene name relE Uniprot ID D2AJT2
Locus tag C1P81_RS25220 Protein ID WP_000421262.1
Coordinates 123722..123997 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A822PWA0
Locus tag C1P81_RS25215 Protein ID WP_011114774.1
Coordinates 123438..123722 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P81_RS25180 119383..120057 + 675 WP_134799904.1 IS66-like element accessory protein TnpA -
C1P81_RS25185 120054..120404 + 351 WP_005048975.1 IS66 family insertion sequence element accessory protein TnpB -
C1P81_RS25190 120401..122002 + 1602 WP_011110603.1 IS66-like element ISSfl3 family transposase -
C1P81_RS25195 122191..122376 + 186 Protein_141 ATP-binding protein -
C1P81_RS25200 122476..122685 - 210 Protein_142 peptidoglycan-binding protein -
C1P81_RS25205 122735..122986 - 252 WP_001381727.1 transporter -
C1P81_RS25215 123438..123722 + 285 WP_011114774.1 ribbon-helix-helix domain-containing protein Antitoxin
C1P81_RS25220 123722..123997 + 276 WP_000421262.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C1P81_RS25235 124976..125929 + 954 Protein_147 IS66 family transposase -
C1P81_RS25240 126465..126683 + 219 WP_000813626.1 type II toxin-antitoxin system antitoxin CcdA -
C1P81_RS25245 126685..126990 + 306 WP_001159860.1 type II toxin-antitoxin system toxin CcdB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ipaH7.8 / ipaH7.8 / ipaH4.5 / ipaH4.5 / ipaH4.5 / ipaH1.4 / ospD3/senA / ospC1 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG / ipaH1.4 / ospE1 / nleE / icsP/sopA / ospB / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ipaH7.8 / ospC2 / ipaH7.8 / ipaH4.5 / ospD3/senA / ospD3/senA / ospC1 / ospC1 1..251323 251323
- inside IScluster/Tn - ospI / ipaH9.8 / ospG 111690..133560 21870


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10766.48 Da        Isoelectric Point: 9.9324

>T95991 WP_000421262.1 NZ_CP026794:123722-123997 [Shigella flexneri]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T95991 NZ_CP026794:123722-123997 [Shigella flexneri]
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAGAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10529.97 Da        Isoelectric Point: 6.2150

>AT95991 WP_011114774.1 NZ_CP026794:123438-123722 [Shigella flexneri]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSW
ADSLSTDHLLPVPR

Download         Length: 285 bp

>AT95991 NZ_CP026794:123438-123722 [Shigella flexneri]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGTCCTGG
GCGGACAGCCTCAGTACTGACCATCTGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PVM3

Antitoxin

Source ID Structure
AlphaFold DB A0A822PWA0

References